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Improved performance of the modified Hodge test with MacConkey agar for screening carbapenemase-producing Gram-negative bacilli

Authors
 Kyungwon Lee  ;  Chang Ki Kim  ;  Dongeun Yong  ;  Seok Hoon Jeong  ;  Jong Hwa Yum  ;  Young Hee Seo  ;  Jean-Denis Docquier  ;  Yunsop Chong 
Citation
 JOURNAL OF MICROBIOLOGICAL METHODS, Vol.83(2) : 149-152, 2010 
Journal Title
 JOURNAL OF MICROBIOLOGICAL METHODS 
ISSN
 0167-7012 
Issue Date
2010
MeSH
Agar ; Anti-Bacterial Agents/pharmacology ; Bacterial Proteins/biosynthesis* ; Culture Media/chemistry ; Gram-Negative Bacteria/drug effects ; Gram-Negative Bacteria/enzymology* ; Gram-Negative Bacterial Infections/microbiology* ; Humans ; Imipenem/pharmacology ; Mass Screening/methods* ; Microbial Sensitivity Tests/methods ; beta-Lactamases/biosynthesis* ; beta-Lactams/pharmacology
Keywords
Modified Hodge test ; Carbapenemase screening ; MacConkey agar
Abstract
The detection of carbapenemases in Gram-negative bacilli is important for optimal patient treatment and to control spread of the resistance. The modified Hodge test can detect carbapenemase-producing Gram-negative bacilli. In this study, we compared the performance of MacConkey agar and Mueller-Hinton agar for metallo-β-lactamase (MBL) and OXA carbapenemase screening. Overall, the performance of Hodge test was better with MacConkey agar due to enhanced release of β-lactamases from the cells in the presence of bile compounds. Concomitant use of the modified Hodge test could resolve most of the problems with uncertain double-disk synergy tests in MBL detection.
Full Text
http://www.sciencedirect.com/science/article/pii/S0167701210002769
DOI
10.1016/j.mimet.2010.08.010
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
Kim, Chang Ki(김창기)
Yong, Dong Eun(용동은) ORCID logo https://orcid.org/0000-0002-1225-8477
Lee, Kyungwon(이경원) ORCID logo https://orcid.org/0000-0003-3788-2134
Jeong, Seok Hoon(정석훈) ORCID logo https://orcid.org/0000-0001-9290-897X
Chong, Yun Sop(정윤섭)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/102357
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