1 115

Cited 26 times in

Culture of human mesenchymal stem cells using electrosprayed porous chitosan microbeads

Authors
 Yu-Jeong Maeng  ;  Sung-Wook Choi  ;  Hyun Ok Kim  ;  Jung-Hyun Kim 
Citation
 JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, Vol.92(3) : 869-876, 2010 
Journal Title
 JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A 
ISSN
 1549-3296 
Issue Date
2010
MeSH
Cell Proliferation ; Cells, Cultured ; Chitosan* ; Humans ; Mesenchymal Stromal Cells/cytology* ; Microscopy, Electron, Scanning ; Microscopy, Fluorescence ; Microspheres
Abstract
The aim of this study was to fabricate porous chitosan microbeads using an electrospraying method into liquid nitrogen, then thawing and refreezing. The microbeads were then used to evaluate their potential for tissue engineering of human mesenchymal stem cells (hMSCs). Scanning electron microscopy (SEM) and a mercury porosimeter were used to show the morphology of the scaffolds formed and to determine their pore size and porosity. As the chitosan concentration increased (0.5, 1, 1.5, and 2 wt %), the diameter of the porous microbead increased from 350 to 890 microm, and the average pore size and the porosity decreased from 65 to 21 microm and 95 to 38%, respectively. The hMSCs were cultured onto the porous microbeads in a spinner flask. SEM images and methyl tetrazolium salt assays at 3, 7, 14, and 21 days of culture revealed that hMSCs had successfully attached and proliferated inside the porous microbeads. This study demonstrated that electrosprayed porous chitosan microbeads can be used as three-dimensional scaffolds for tissue engineering.
Full Text
http://onlinelibrary.wiley.com/doi/10.1002/jbm.a.32417/abstract
DOI
10.1002/jbm.a.32417
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
Kim, Hyun Ok(김현옥) ORCID logo https://orcid.org/0000-0002-4964-1963
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/102187
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse