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The transcription factor snail regulates osteogenic differentiation by repressing Runx2 expression

Authors
 Su Jin Park  ;  Seung-Hyun Jung  ;  Gadi Jogeswar  ;  Hyun-Mo Ryoo  ;  Jong In Yook  ;  Han Seok Choi  ;  Yumie Rhee  ;  Cheol-Hee Kim  ;  Sung-Kil Lim 
Citation
 Bone, Vol.46(6) : 1498-1507, 2010 
Journal Title
 Bone 
ISSN
 8756-3282 
Issue Date
2010
MeSH
Animals ; Blotting, Western ; Cell Differentiation/genetics ; Cell Differentiation/physiology* ; Cell Line ; Cell Proliferation ; Chromatin Immunoprecipitation ; Core Binding Factor Alpha 1 Subunit/genetics ; Core Binding Factor Alpha 1 Subunit/metabolism* ; Electrophoretic Mobility Shift Assay ; Flow Cytometry ; Mice ; Mutagenesis, Site-Directed ; Osteoblasts/cytology* ; Osteoblasts/metabolism* ; RNA Interference ; Reverse Transcriptase Polymerase Chain Reaction ; Snail Family Transcription Factors ; Transcription Factors/genetics ; Transcription Factors/metabolism*
Keywords
Snail ; Runx2 ; E-box ; Osteogenic differentiation
Abstract
Osteoblasts originate from mesenchymal stem cells by the coordinated activities of different signaling pathways that regulate the expression of osteoblast-specific genes. Runt-related transcription factor 2 (Runx2) is the master transcription factor for osteoblast differentiation. Despite the importance of Runx2 in the developing skeleton, how Runx2 expression is regulated remains a pivotal question. Snail, a zinc finger transcription factor, is essential for triggering epithelial-to-mesenchymal transitions (EMTs) during embryonic development and tumor progression. Here, we report that Runx2 expression is significantly up- or down-regulated relative to Snail expression. We demonstrate that Snail binds to the Runx2 promoter and that repression of Runx2 transcription by Snail is dependent on specific E-box sequence within the promoter. With antisense morpholino oligonucleotide (MO)-mediated knockdown of Snail expression in zebrafish, we observed alterations in osteogenic potential. These results indicate that Snail plays a crucial role in osteogenic differentiation by acting as a direct Runx2 repressor.
Full Text
http://www.sciencedirect.com/science/article/pii/S8756328210004874
DOI
10.1016/j.bone.2010.02.027
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Oral Pathology (구강병리학교실) > 1. Journal Papers
5. Research Institutes (연구소) > Institute of Endocrinology (내분비연구소) > 1. Journal Papers
Yonsei Authors
Gadi, Jogeswar(가디죠게스)
Park, Su Jin(박수진)
Yook, Jong In(육종인) ORCID logo https://orcid.org/0000-0002-7318-6112
Rhee, Yumie(이유미) ORCID logo https://orcid.org/0000-0003-4227-5638
Lim, Sung Kil(임승길)
Choi, Han Seok(최한석)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/102050
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