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Role and cellular source of nicotinamide adenine dinucleotide phosphate oxidase in hepatic fibrosis

DC FieldValueLanguage
dc.contributor.author백용한-
dc.date.accessioned2015-04-23T17:05:31Z-
dc.date.available2015-04-23T17:05:31Z-
dc.date.issued2010-
dc.identifier.issn0270-9139-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/101804-
dc.description.abstractReactive oxygen species (ROS) generated by nicotinamide adenine dinucleotide phosphate oxidase (NOX) is required for liver fibrosis. This study investigates the role of NOX in ROS production and the differential contribution of NOX from bone marrow (BM)-derived and non-BM-derived liver cells. Hepatic fibrosis was induced by bile duct ligation (BDL) for 21 days or by methionine-choline-deficient (MCD) diet for 10 weeks in wild-type (WT) mice and mice deficient in p47phox (p47phox knockout [KO]), a component of NOX. The p47phox KO chimeric mice were generated by the combination of liposomal clodronate injection, irradiation, and BM transplantation of p47phox KO BM into WT recipients and vice versa. Upon BDL, chimeric mice with p47phox KO BM-derived cells, including Kupffer cells, and WT endogenous liver cells showed a ∼25% reduction of fibrosis, whereas chimeric mice with WT BM-derived cells and p47phox KO endogenous liver cells, including hepatic stellate cells, showed a ∼60% reduction of fibrosis. In addition, p47phox KO compared to WT mice treated with an MCD diet showed no significant changes in steatosis and hepatocellular injury, but a ∼50% reduction in fibrosis. Cultured WT and p47phox KO hepatocytes treated with free fatty acids had a similar increase in lipid accumulation. Free fatty acids promoted a 1.5-fold increase in ROS production both in p47phox KO and in WT hepatocytes. CONCLUSION: NOX in both BM-derived and non-BM-derived cells contributes to liver fibrosis. NOX does not play a role in experimental steatosis and the generation of ROS in hepatocytes, but exerts a key role in fibrosis.-
dc.description.statementOfResponsibilityopen-
dc.format.extent1420~1430-
dc.relation.isPartOfHEPATOLOGY-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAnimals-
dc.subject.MESHBile Ducts/surgery-
dc.subject.MESHBone Marrow Transplantation-
dc.subject.MESHCarbon Tetrachloride Poisoning/complications-
dc.subject.MESHCholine Deficiency/physiopathology-
dc.subject.MESHHepatic Stellate Cells/enzymology-
dc.subject.MESHKupffer Cells/enzymology-
dc.subject.MESHLigation-
dc.subject.MESHLipid Peroxidation-
dc.subject.MESHLiver/cytology*-
dc.subject.MESHLiver/enzymology-
dc.subject.MESHLiver Cirrhosis/enzymology*-
dc.subject.MESHLiver Cirrhosis/etiology-
dc.subject.MESHLiver Cirrhosis/physiopathology-
dc.subject.MESHMale-
dc.subject.MESHMethionine/deficiency-
dc.subject.MESHMice-
dc.subject.MESHMice, Inbred C57BL-
dc.subject.MESHMice, Knockout-
dc.subject.MESHNADPH Oxidases/metabolism*-
dc.subject.MESHNADPH Oxidases/physiology-
dc.subject.MESHReactive Oxygen Species/metabolism-
dc.subject.MESHTransplantation Chimera-
dc.titleRole and cellular source of nicotinamide adenine dinucleotide phosphate oxidase in hepatic fibrosis-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Internal Medicine (내과학)-
dc.contributor.googleauthorSamuele De Minicis-
dc.contributor.googleauthorEkihiro Seki-
dc.contributor.googleauthorYong-Han Paik-
dc.contributor.googleauthorChristoph H. Österreicher-
dc.contributor.googleauthorYuzo Kodama-
dc.contributor.googleauthorJohannes Kluwe-
dc.contributor.googleauthorLuciano Torozzi-
dc.contributor.googleauthorKatsumi Miyai-
dc.contributor.googleauthorAntonio Benedetti-
dc.contributor.googleauthorRobert F. Schwabe-
dc.contributor.googleauthorDavid A. Brenner-
dc.identifier.doi10.1002/hep.23804-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA01829-
dc.relation.journalcodeJ00985-
dc.identifier.eissn1527-3350-
dc.identifier.pmid20690191-
dc.contributor.alternativeNamePaik, Yong Han-
dc.contributor.affiliatedAuthorPaik, Yong Han-
dc.citation.volume52-
dc.citation.number4-
dc.citation.startPage1420-
dc.citation.endPage1430-
dc.identifier.bibliographicCitationHEPATOLOGY, Vol.52(4) : 1420-1430, 2010-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers

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