Na와 F 원소가 칼슘 포스페이트 글라스의 용해도 및 세포 활성도에 미치는 영향

Abstract

The development of phosphate glasses for clinical applications has attracted much interest because their chemical and physical properties make them suitable for use as bone-graft materials. We prepared various compositions of CaO-P2O5-CaF2-Na2O glass system by conventional melting process. The objective of this work was to investigate the effect of sodium and fluorine elements on calcium phosphate glasses for bone regeneration by measuring solubility and biological response. Atomic ratio of (Ca+Na)/P and a content of P2O5 were fixed at 0.6 and 45.45 mol%, respectively. To measure solubility, the pellet was kept into 30 mL of distilled water. At the same time as the solubility measurements, the pH of the solution was also measured. Ca, P, and Na ion dissolution were measured by Inductively Coupled Plasma Atomic Emission Spectrophotometer and the release of F ion was determined using Fluoride Ion Selective Electrode. Biological response of glass extracts was measured using WST-1 method. Glasses with high content of sodium oxides showed larger solubility rate, but in case of glasses with F element, there were few changes. The dissolved amount of Ca and P ion was related to solubility; with high solubility, the dissolved amount was increased. Except for glass with CaF2 and Na2O content of each 9 mol%, with increasing added oxides content and time, the pH value tended to be lower. pH values decreased with P ion content increasing. The viability of MG 63 cell in glass extracts of 2 weeks showed above 90%.