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Fast and Efficient Isolation of Mouse Bone Marrow-Derived Mesenchymal Stem Cells by Using a Biocompatible Polymer
DC Field | Value | Language |
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dc.contributor.author | 김한수 | - |
dc.contributor.author | 김현옥 | - |
dc.date.accessioned | 2015-04-23T17:02:39Z | - |
dc.date.available | 2015-04-23T17:02:39Z | - |
dc.date.issued | 2010 | - |
dc.identifier.issn | 1738-2696 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/101716 | - |
dc.description.abstract | Mesenchymal stem cells (MSCs) differentiate into bone, fat, cartilage, tendon, and other organ progenitor cells. The rarity of MSCs in bone marrow necessitates fast and efficient isolation and/or in vitro expansion prior to clinical and biomedical applications. Previously, we reported that UV-exposed diphenylamino-s-triazine bridged p-phenylene vinylene (DTOPV-UV) with a hydrophilic and negative surface-containing carboxyl group is highly biocompatible and provides a substrate for efficient human bone marrow-derived MSC attachment. In this study, we applied this polymeric film to early adhesion and enrichment of MSCs from mouse bone marrow. With its high protein- binding capacity, DTOPV-UV film was more efficient in early capture of adherent bone marrow cells than conventional tissue culture polystyrene (TCPS). Cell binding to DTOPV-UV reached full capacity within 1 hr, whereas cell attachment to TCPS gradually increased over time. The isolated and culture-expanded MSCs from mouse bone marrow displayed typical morphology, phenotype, and differentiation into osteoblasts, adipocytes, and chondrocytes. Here, we demonstrate a novel method for isolating MSCs from mouse bone marrow using a biocompatible polymer. This method will aid the development of rapid and efficient isolation and in vitro expansion protocols for rare adherent cells. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 443~451 | - |
dc.relation.isPartOf | TISSUE ENGINEERING AND REGENERATIVE MEDICINE | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | Fast and Efficient Isolation of Mouse Bone Marrow-Derived Mesenchymal Stem Cells by Using a Biocompatible Polymer | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Laboratory Medicine (진단검사의학) | - |
dc.contributor.googleauthor | Han-Soo Kim | - |
dc.contributor.googleauthor | June Seok Heo | - |
dc.contributor.googleauthor | Jungmok You | - |
dc.contributor.googleauthor | Teahoon Park | - |
dc.contributor.googleauthor | Youjeong Choi | - |
dc.contributor.googleauthor | Eunkyoung Kim | - |
dc.contributor.googleauthor | Hyun Ok Kim | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01100 | - |
dc.contributor.localId | A01122 | - |
dc.relation.journalcode | J02734 | - |
dc.identifier.eissn | 2212-5469 | - |
dc.subject.keyword | adhesion | - |
dc.subject.keyword | mesenchymal stem cell | - |
dc.subject.keyword | bone marrow | - |
dc.subject.keyword | carboxyl group | - |
dc.contributor.alternativeName | Kim, Han Soo | - |
dc.contributor.alternativeName | Kim, Hyun Ok | - |
dc.contributor.affiliatedAuthor | Kim, Han Soo | - |
dc.contributor.affiliatedAuthor | Kim, Hyun Ok | - |
dc.citation.volume | 7 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 443 | - |
dc.citation.endPage | 451 | - |
dc.identifier.bibliographicCitation | TISSUE ENGINEERING AND REGENERATIVE MEDICINE, Vol.7(4) : 443-451, 2010 | - |
dc.identifier.rimsid | 46685 | - |
dc.type.rims | ART | - |
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