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Fast and Efficient Isolation of Mouse Bone Marrow-Derived Mesenchymal Stem Cells by Using a Biocompatible Polymer

DC FieldValueLanguage
dc.contributor.author김한수-
dc.contributor.author김현옥-
dc.date.accessioned2015-04-23T17:02:39Z-
dc.date.available2015-04-23T17:02:39Z-
dc.date.issued2010-
dc.identifier.issn1738-2696-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/101716-
dc.description.abstractMesenchymal stem cells (MSCs) differentiate into bone, fat, cartilage, tendon, and other organ progenitor cells. The rarity of MSCs in bone marrow necessitates fast and efficient isolation and/or in vitro expansion prior to clinical and biomedical applications. Previously, we reported that UV-exposed diphenylamino-s-triazine bridged p-phenylene vinylene (DTOPV-UV) with a hydrophilic and negative surface-containing carboxyl group is highly biocompatible and provides a substrate for efficient human bone marrow-derived MSC attachment. In this study, we applied this polymeric film to early adhesion and enrichment of MSCs from mouse bone marrow. With its high protein- binding capacity, DTOPV-UV film was more efficient in early capture of adherent bone marrow cells than conventional tissue culture polystyrene (TCPS). Cell binding to DTOPV-UV reached full capacity within 1 hr, whereas cell attachment to TCPS gradually increased over time. The isolated and culture-expanded MSCs from mouse bone marrow displayed typical morphology, phenotype, and differentiation into osteoblasts, adipocytes, and chondrocytes. Here, we demonstrate a novel method for isolating MSCs from mouse bone marrow using a biocompatible polymer. This method will aid the development of rapid and efficient isolation and in vitro expansion protocols for rare adherent cells.-
dc.description.statementOfResponsibilityopen-
dc.format.extent443~451-
dc.relation.isPartOfTISSUE ENGINEERING AND REGENERATIVE MEDICINE-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.titleFast and Efficient Isolation of Mouse Bone Marrow-Derived Mesenchymal Stem Cells by Using a Biocompatible Polymer-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Laboratory Medicine (진단검사의학)-
dc.contributor.googleauthorHan-Soo Kim-
dc.contributor.googleauthorJune Seok Heo-
dc.contributor.googleauthorJungmok You-
dc.contributor.googleauthorTeahoon Park-
dc.contributor.googleauthorYoujeong Choi-
dc.contributor.googleauthorEunkyoung Kim-
dc.contributor.googleauthorHyun Ok Kim-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA01100-
dc.contributor.localIdA01122-
dc.relation.journalcodeJ02734-
dc.identifier.eissn2212-5469-
dc.subject.keywordadhesion-
dc.subject.keywordmesenchymal stem cell-
dc.subject.keywordbone marrow-
dc.subject.keywordcarboxyl group-
dc.contributor.alternativeNameKim, Han Soo-
dc.contributor.alternativeNameKim, Hyun Ok-
dc.contributor.affiliatedAuthorKim, Han Soo-
dc.contributor.affiliatedAuthorKim, Hyun Ok-
dc.citation.volume7-
dc.citation.number4-
dc.citation.startPage443-
dc.citation.endPage451-
dc.identifier.bibliographicCitationTISSUE ENGINEERING AND REGENERATIVE MEDICINE, Vol.7(4) : 443-451, 2010-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers

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