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Simple and highly effective DNA extraction methods from old skeletal remains using silica columns

DC Field Value Language
dc.contributor.author김나영-
dc.contributor.author박명진-
dc.contributor.author신경진-
dc.contributor.author심정은-
dc.contributor.author이환영-
dc.date.accessioned2015-04-23T17:02:09Z-
dc.date.available2015-04-23T17:02:09Z-
dc.date.issued2010-
dc.identifier.issn1872-4973-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/101701-
dc.description.abstractThe recovery of DNA data from old skeletal remains is often difficult due to degraded and very low yield of extracted DNA and the presence of PCR inhibitors. Herein, we compared several silica-based DNA extraction methods from artificially degraded DNA, DNA with PCR inhibitors and DNA from old skeletal remains using quantitative real-time PCR. We present a modified large-scale silica-based extraction combined with complete demineralization, that enables maximum DNA recovery and efficient elimination of PCR inhibitors. This is performed with high concentration of EDTA solution for demineralization of bone powder followed by QIAamp spin columns and buffers from the QIAquick PCR purification kit. We have successfully used this modified technique to perform STR analysis for 55-year-old skeletal remains. The results of this study will contribute to solve the forensic cases dealing with skeletal remains.-
dc.description.statementOfResponsibilityopen-
dc.format.extent275~280-
dc.relation.isPartOfFORENSIC SCIENCE INTERNATIONAL-GENETICS-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHBone and Bones/metabolism*-
dc.subject.MESHDNA/isolation & purification*-
dc.subject.MESHHumans-
dc.subject.MESHMicrosatellite Repeats-
dc.subject.MESHPolymerase Chain Reaction/methods-
dc.titleSimple and highly effective DNA extraction methods from old skeletal remains using silica columns-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Forensic Medicine (법의학)-
dc.contributor.googleauthorHwan Young Lee-
dc.contributor.googleauthorMyung Jin Park-
dc.contributor.googleauthorNa Young Kim-
dc.contributor.googleauthorJeong Eun Sim-
dc.contributor.googleauthorWoo Ick Yang-
dc.contributor.googleauthorKyoung-Jin Shin-
dc.identifier.doi10.1016/j.fsigen.2009.10.014-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA01456-
dc.contributor.localIdA02085-
dc.contributor.localIdA02210-
dc.contributor.localIdA03335-
dc.contributor.localIdA00349-
dc.relation.journalcodeJ00905-
dc.identifier.eissn1878-0326-
dc.identifier.pmid20457067-
dc.identifier.urlhttp://www.sciencedirect.com/science/article/pii/S1872497310000050-
dc.subject.keywordDNA extraction-
dc.subject.keywordSkeletal remains-
dc.subject.keywordComplete demineralization-
dc.subject.keywordSilica-based column-
dc.subject.keywordPolymerase chain reaction inhibitor-
dc.contributor.alternativeNameKim, Na Young-
dc.contributor.alternativeNamePark, Myung Jin-
dc.contributor.alternativeNameShin, Kyoung Jin-
dc.contributor.alternativeNameSim, Jeong Eun-
dc.contributor.alternativeNameLee, Hwan Young-
dc.contributor.affiliatedAuthorPark, Myung Jin-
dc.contributor.affiliatedAuthorShin, Kyoung Jin-
dc.contributor.affiliatedAuthorSim, Jeong Eun-
dc.contributor.affiliatedAuthorLee, Hwan Young-
dc.contributor.affiliatedAuthorKim, Na Young-
dc.citation.volume4-
dc.citation.number5-
dc.citation.startPage275-
dc.citation.endPage280-
dc.identifier.bibliographicCitationFORENSIC SCIENCE INTERNATIONAL-GENETICS, Vol.4(5) : 275-280, 2010-
dc.identifier.rimsid46674-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Forensic Medicine (법의학과) > 1. Journal Papers

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