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Simple and highly effective DNA extraction methods from old skeletal remains using silica columns

Authors
 Hwan Young Lee  ;  Myung Jin Park  ;  Na Young Kim  ;  Jeong Eun Sim  ;  Woo Ick Yang  ;  Kyoung-Jin Shin 
Citation
 FORENSIC SCIENCE INTERNATIONAL-GENETICS, Vol.4(5) : 275-280, 2010 
Journal Title
FORENSIC SCIENCE INTERNATIONAL-GENETICS
ISSN
 1872-4973 
Issue Date
2010
MeSH
Bone and Bones/metabolism* ; DNA/isolation & purification* ; Humans ; Microsatellite Repeats ; Polymerase Chain Reaction/methods
Keywords
DNA extraction ; Skeletal remains ; Complete demineralization ; Silica-based column ; Polymerase chain reaction inhibitor
Abstract
The recovery of DNA data from old skeletal remains is often difficult due to degraded and very low yield of extracted DNA and the presence of PCR inhibitors. Herein, we compared several silica-based DNA extraction methods from artificially degraded DNA, DNA with PCR inhibitors and DNA from old skeletal remains using quantitative real-time PCR. We present a modified large-scale silica-based extraction combined with complete demineralization, that enables maximum DNA recovery and efficient elimination of PCR inhibitors. This is performed with high concentration of EDTA solution for demineralization of bone powder followed by QIAamp spin columns and buffers from the QIAquick PCR purification kit. We have successfully used this modified technique to perform STR analysis for 55-year-old skeletal remains. The results of this study will contribute to solve the forensic cases dealing with skeletal remains.
Full Text
http://www.sciencedirect.com/science/article/pii/S1872497310000050
DOI
10.1016/j.fsigen.2009.10.014
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Forensic Medicine (법의학과) > 1. Journal Papers
Yonsei Authors
Kim, Na Young(김나영)
Park, Myung Jin(박명진)
Shin, Kyoung Jin(신경진) ORCID logo https://orcid.org/0000-0002-1059-9665
Sim, Jeong Eun(심정은)
Lee, Hwan Young(이환영)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/101701
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