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Oct4 and Nanog expression is associated with early stages of pancreatic carcinogenesis

 Jing Wen  ;  Jeong Youp Park  ;  Kyung Hwa Park  ;  Hye Won Chung  ;  Seungmin Bang  ;  Seung Woo Park  ;  Si Young Song 
 PANCREAS, Vol.39(5) : 622-626, 2010 
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Animals ; Carcinoma, Pancreatic Ductal/genetics ; Carcinoma, Pancreatic Ductal/metabolism* ; Carcinoma, Pancreatic Ductal/pathology ; Cell Transformation, Neoplastic/genetics ; Cell Transformation, Neoplastic/metabolism* ; Cell Transformation, Neoplastic/pathology ; Cricetinae ; Genes, ras ; Homeodomain Proteins/analysis ; Homeodomain Proteins/biosynthesis* ; Humans ; Mutation ; Nanog Homeobox Protein ; Nitrosamines/pharmacology ; Octamer Transcription Factor-3/analysis ; Octamer Transcription Factor-3/biosynthesis* ; Pancreatic Neoplasms/genetics ; Pancreatic Neoplasms/metabolism* ; Pancreatic Neoplasms/pathology
OBJECTIVE: To characterize the role of Oct4 and Nanog, two important homeobox transcription factors of embryonic development, in pancreatic carcinogenesis. METHODS: Using a tissue microarray of human pancreatic carcinoma and adjacent noncancerous tissues as well as the N-nitrosobis(2-oxopropyl)amine-induced Syrian golden hamster pancreatic cancer model, we characterized the expression of Oct4 and Nanog. The presence of K-ras mutation with the time course of carcinogenesis in hamster model was also evaluated. RESULTS: Oct4 expression in metaplastic ducts was significantly stronger than in normal acini and pancreatic carcinoma (P < 0.05). Of 24 cases, 19 (79.2%) showed a strong Oct4 expression in metaplastic ducts. In contrast, only 6 (19.4%) of 31 cancer tissues and 3 (16.7%) of 18 noncancer tissues showed a strong Oct4 expression. Nanog also showed similar patterns as Oct4. Restriction fragment length polymorphism-polymerase chain reaction showed the overt K-ras mutation after the expression of Oct4 in the hamster model. CONCLUSIONS: The strong expression of Oct4 and Nanog in metaplastic ducts and Oct4 expression preceding Ras mutation suggests that these homeobox transcription factors are associated with the early stage of pancreatic cancer carcinogenesis and may play an important role in that process.
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1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Park, Seung Woo(박승우) ORCID logo https://orcid.org/0000-0001-8230-964X
Park, Jeong Youp(박정엽) ORCID logo https://orcid.org/0000-0003-0110-8606
Bang, Seungmin(방승민) ORCID logo https://orcid.org/0000-0001-5209-8351
Song, Si Young(송시영) ORCID logo https://orcid.org/0000-0002-1417-4314
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