Cited 19 times in
Insulin-like growth factor binding protein-3 induces G1 cell cycle arrest with inhibition of cyclin-dependent kinase 2 and 4 in MCF-7 human breast cancer cells.
DC Field | Value | Language |
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dc.contributor.author | 이선우 | - |
dc.contributor.author | 채현욱 | - |
dc.contributor.author | 김덕희 | - |
dc.contributor.author | 김호성 | - |
dc.date.accessioned | 2015-04-23T16:47:51Z | - |
dc.date.available | 2015-04-23T16:47:51Z | - |
dc.date.issued | 2010 | - |
dc.identifier.issn | 0018-5043 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/101244 | - |
dc.description.abstract | Insulin-like growth factor binding protein (IGFBP)-3 has been shown to potently inhibit proliferation of various cell types in an insulin-like growth factor (IGF)-independent manner. We have previously shown that IGFBP-3 induces apoptosis in an IGF-independent manner through the activation of caspases involved in a death receptor-mediated pathway in MCF-7 human breast cancer cells. In the present study, we present further evidence that IGFBP-3 inhibits cell proliferation through the induction of cell cycle arrest in the same cell line. Induction of IGFBP-3 in MCF-7 cells inhibited cell proliferation whereas presence of small interfering RNA against IGFBP-3 abolished cell inhibitory effect of IGFBP-3, suggesting that the observed growth inhibition is specific. Flow cytometry analysis showed that induced expression of IGFBP-3 led to an arrest of the cell cycle in G1-S phase. Western immunoblot analysis showed a significant decrease in the levels of the cell cycle-regulated proteins such as cyclin D1, cyclin D3, cyclin E, cyclin A, cyclin-dependent kinase (CDK) 2, CDK4, retinoblastoma protein (pRB), and phosph-pRB, suggesting a possible mechanism for cell cycle arrest by IGFBP-3. Northern blot analysis and real-time quantitative PCR demonstrated a significant decrease in gene expression of cyclin D1. Additional phosphorylation assay showed that IGFBP-3 decreased the phosphorylation activity of CDK2 and CDK4. These results show that cellular production of IGFBP-3 leads to G1 cell cycle arrest with inhibition of CDK2 and CDK4. Taken together, IGFBP-3 exerts its growth inhibitory action through not only induction of apoptosis but also the G1 cell cycle arrest in human breast cancer cells. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 165~172 | - |
dc.relation.isPartOf | HORMONE AND METABOLIC RESEARCH | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Breast Neoplasms/enzymology* | - |
dc.subject.MESH | Breast Neoplasms/genetics | - |
dc.subject.MESH | Breast Neoplasms/pathology* | - |
dc.subject.MESH | Cell Line, Tumor | - |
dc.subject.MESH | Cell Proliferation/drug effects | - |
dc.subject.MESH | Cyclin D1/genetics | - |
dc.subject.MESH | Cyclin D1/metabolism | - |
dc.subject.MESH | Cyclin-Dependent Kinase 2/antagonists & inhibitors* | - |
dc.subject.MESH | Cyclin-Dependent Kinase 2/metabolism | - |
dc.subject.MESH | Cyclin-Dependent Kinase 4/antagonists & inhibitors* | - |
dc.subject.MESH | Cyclin-Dependent Kinase 4/metabolism | - |
dc.subject.MESH | Cyclin-Dependent Kinase Inhibitor Proteins/metabolism | - |
dc.subject.MESH | Ecdysterone/analogs & derivatives | - |
dc.subject.MESH | Ecdysterone/pharmacology | - |
dc.subject.MESH | Female | - |
dc.subject.MESH | G1 Phase*/drug effects | - |
dc.subject.MESH | Gene Expression Regulation, Neoplastic/drug effects | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Insulin-Like Growth Factor Binding Protein 3 | - |
dc.subject.MESH | Insulin-Like Growth Factor Binding Proteins/metabolism* | - |
dc.subject.MESH | RNA, Messenger/genetics | - |
dc.subject.MESH | RNA, Messenger/metabolism | - |
dc.title | Insulin-like growth factor binding protein-3 induces G1 cell cycle arrest with inhibition of cyclin-dependent kinase 2 and 4 in MCF-7 human breast cancer cells. | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Pediatrics (소아과학) | - |
dc.contributor.googleauthor | H.-S. Kim | - |
dc.contributor.googleauthor | W. J. Lee | - |
dc.contributor.googleauthor | S. W. Lee | - |
dc.contributor.googleauthor | H.-W. Chae | - |
dc.contributor.googleauthor | D. H. Kim | - |
dc.contributor.googleauthor | Y. Oh | - |
dc.identifier.doi | 10.1055/s-0029-1243190 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A02858 | - |
dc.contributor.localId | A04026 | - |
dc.contributor.localId | A00378 | - |
dc.contributor.localId | A01184 | - |
dc.relation.journalcode | J00999 | - |
dc.identifier.eissn | 1439-4286 | - |
dc.identifier.pmid | 19960406 | - |
dc.identifier.url | https://www.thieme-connect.de/DOI/DOI?10.1055/s-0029-1243190 | - |
dc.contributor.alternativeName | Lee, Sun Woo | - |
dc.contributor.alternativeName | Chae, Hyun Wook | - |
dc.contributor.alternativeName | Kim, Duk Hee | - |
dc.contributor.alternativeName | Kim, Ho Seong | - |
dc.contributor.affiliatedAuthor | Lee, Sun Woo | - |
dc.contributor.affiliatedAuthor | Chae, Hyun Wook | - |
dc.contributor.affiliatedAuthor | Kim, Duk Hee | - |
dc.contributor.affiliatedAuthor | Kim, Ho Seong | - |
dc.citation.volume | 42 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 165 | - |
dc.citation.endPage | 172 | - |
dc.identifier.bibliographicCitation | HORMONE AND METABOLIC RESEARCH, Vol.42(3) : 165-172, 2010 | - |
dc.identifier.rimsid | 52158 | - |
dc.type.rims | ART | - |
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