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Functional characterization of the IlpA protein of Vibrio vulnificus as an adhesin and its role in bacterial pathogenesis

Authors
 Kyung-Jo Lee  ;  Na Yeon Lee  ;  Yang-Soo Han  ;  Juri Kim  ;  Kyu-Ho Lee  ;  Soon-Jung Park 
Citation
 INFECTION AND IMMUNITY, Vol.78(6) : 2408-2417, 2010 
Journal Title
 INFECTION AND IMMUNITY 
ISSN
 0019-9567 
Issue Date
2010
MeSH
Adhesins, Bacterial/genetics ; Adhesins, Bacterial/physiology* ; Bacterial Adhesion ; Cell Line ; Epithelial Cells/microbiology ; Gene Deletion ; Genetic Complementation Test ; Humans ; Vibrio vulnificus/pathogenicity*
Abstract
Vibrio vulnificus is a Gram-negative bacterium that causes a fatal septicemia. One of its virulence factors is a membrane-bound lipoprotein, IlpA, which can induce cytokine production in human immune cells. In the present study, the role of IlpA as an adhesion molecule was investigated. An ilpA-deleted V. vulnificus mutant showed significantly decreased adherence to INT-407 human intestinal epithelial cells, which in turn resulted in reduced cytotoxicity. The DeltailpA mutant recovered the adherence ability of the wild type by complementation in trans with the intact ilpA gene. In addition, pretreatment of V. vulnificus with anti-IlpA polyclonal antibodies resulted in a significant reduction of bacterial adherence. To localize the domain of IlpA required for cytoadherence, three truncated recombinant IlpA polypeptides were constructed and tested for the ability to adhere to human cells by a ligand-binding immunoblot assay and fluorescence microscopy. The polypeptide containing the carboxy (C)-terminal hydrophilic domain exhibited direct binding to INT-407 cells. Therefore, the C-terminal domain of IlpA allows this protein to be an adhesion molecule of V. vulnificus.
Files in This Item:
T201001845.pdf Download
DOI
10.1128/IAI.01194-09
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Environmental Medical Biology (환경의생물학교실) > 1. Journal Papers
Yonsei Authors
Kim, Ju Ri(김주리) ORCID logo https://orcid.org/0000-0001-8270-7584
Park, Soon Jung(박순정) ORCID logo https://orcid.org/0000-0002-0423-1944
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/101218
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