244 417

Cited 7 times in

Performance Characteristics of the UniCel DxI 800 Immunoassay for the Maternal Serum Quadruple Test, Including Median Values for Each Week of Gestation, in Korean Women

Authors
 Jeong Hoon Lee  ;  Yongjung Park  ;  Borum Suh  ;  Seon-Mi Song  ;  Oh Hun Kwon  ;  Jeong-Ho Kim 
Citation
 KOREAN JOURNAL OF LABORATORY MEDICINE , Vol.30(2) : 126-132, 2010 
Journal Title
 KOREAN JOURNAL OF LABORATORY MEDICINE 
ISSN
 1598-6535 
Issue Date
2010
MeSH
Biomarkers/blood ; Chorionic Gonadotropin/blood ; Enzyme-Linked Immunosorbent Assay ; Estriol/blood ; Female ; Gestational Age ; Humans ; Immunoassay/instrumentation ; Immunoassay/methods* ; Inhibins/blood ; Pregnancy ; Pregnancy Trimester, Second ; Prenatal Diagnosis* ; Reference Values ; Republic of Korea ; alpha-Fetoproteins/analysis
Abstract
BACKGROUND: Maternal serum prenatal quadruple screening includes testing for alpha-fetoprotein (AFP), human chorionic gonadotrophin (hCG), unconjugated estriol (uE3), and dimeric inhibin A (DIA). We evaluated quadruple screening using an automated platform and looked for any ethnic differences in the median values of each marker. METHODS: We measured the concentrations of each quadruple test analyte using the UniCel DxI 800 system (Beckman Coulter, USA) in 788 Korean mid-trimester maternal serum samples and calculated their median values using Benetech software (Benetech, Canada). We also compared the results with those obtained using the Immulite 2000 assay (Siemens Healthcare Diagnostics, USA) or ELISA (DSL, USA) in 442 samples. RESULTS: We obtained mid-trimester median values for each marker. The following are the comparative results for each test using the Immulite 2000 assay or ELISA (x) and the UniCel DxI 800 immunoassay (y): AFP, y=1.10x+0.01, r=0.925; uE3, y=0.28x+0.24, r=0.885; hCG, y=1.22x-3047.8, r=0.944; and DIA, y=0.86x+15.31, r=0.833. Assay results for each of the four markers showed good correlations. However, significant biases necessitated new median calculations of prenatal risk estimates in all four tests. CONCLUSIONS: We established gestational age-specific second-trimester median values for four markers in Korean samples using the UniCel DxI 800 immunoassay system. Despite significant bias, there were good correlations between the results obtained using the UniCel DxI 800 immunoassay and those obtained using the Immulite 2000 assay.
Files in This Item:
T201001826.pdf Download
DOI
10.3343/kjlm.2010.30.2.126
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
Kwon, Oh Hun(권오헌)
Kim, Jeong Ho(김정호) ORCID logo https://orcid.org/0000-0003-2479-0548
Park, Yong Jung(박용정)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/101203
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse