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Cited 64 times in

Molecular proteomics imaging of tumor interfaces by mass spectrometry

DC Field Value Language
dc.contributor.author강숙희-
dc.contributor.author심효섭-
dc.contributor.author윤주헌-
dc.contributor.author조남훈-
dc.date.accessioned2015-04-23T16:33:05Z-
dc.date.available2015-04-23T16:33:05Z-
dc.date.issued2010-
dc.identifier.issn1535-3893-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/100789-
dc.description.abstractThe specific molecular profiles of ovarian cancer interface zones (IZ), the region between tumors and normal tissues, were evaluated using a new method involving matrix-assisted laser desorption/ionization (MALDI)-imaging mass spectrometry (IMS). We analyzed three ovarian serous carcinomas using MALDI-IMS. Principal component analysis (PCA) was used to evaluate the quality of tissue spatial features based on MALDI-IMS, and for analysis of large data sets of MALDI-IMS. Two-dimensional gel electrophoresis and fluorescence microscopy were used to verify interface-specific proteins. Unique profiles were identified for the tumors, the normal zone, and the IZ. Through MALDI analysis, two interface-specific proteins, plastin 2 and peroxiredoxin 1 (PRDX 1), were identified as differentially regulated between zones. Fluorescence microscopy revealed high expression levels of plastin 2 and PRDX 1 along the IZ of ovarian tumors. This comparative proteomics study using tissue MALDI-IMS suggested that the IZ is different from the adjacent tumor and normal zones, and that plastin 2 and PRDX 1 may be interface markers specific to ovarian tumors-
dc.description.statementOfResponsibilityopen-
dc.format.extent1157~1164-
dc.relation.isPartOfJOURNAL OF PROTEOME RESEARCH-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHElectrophoresis, Gel, Two-Dimensional-
dc.subject.MESHFemale-
dc.subject.MESHHumans-
dc.subject.MESHImmunohistochemistry-
dc.subject.MESHMicroscopy, Fluorescence-
dc.subject.MESHOvarian Neoplasms/chemistry*-
dc.subject.MESHPrincipal Component Analysis-
dc.subject.MESHProteomics*-
dc.subject.MESHSpectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods*-
dc.titleMolecular proteomics imaging of tumor interfaces by mass spectrometry-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Pathology (병리학)-
dc.contributor.googleauthorSuki Kang-
dc.contributor.googleauthorHyo Sup Shim-
dc.contributor.googleauthorJong Sik Lee-
dc.contributor.googleauthorDong Su Kim-
dc.contributor.googleauthorHak Yong Kim-
dc.contributor.googleauthorSeong Hyun Hong-
dc.contributor.googleauthorPan Soo Kim-
dc.contributor.googleauthorJoo Heon Yoon-
dc.contributor.googleauthorNam Hoon Cho-
dc.identifier.doi10.1021/pr900666q-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA00044-
dc.contributor.localIdA02219-
dc.contributor.localIdA02604-
dc.contributor.localIdA03812-
dc.relation.journalcodeJ01720-
dc.identifier.eissn1535-3907-
dc.identifier.pmid19821573-
dc.identifier.urlhttp://pubs.acs.org/doi/abs/10.1021/pr900666q-
dc.subject.keyword2-dimensional gel electrophoresis-
dc.subject.keywordinterface-
dc.subject.keywordMALDI-IMS-
dc.subject.keywordovarian cancer-
dc.contributor.alternativeNameKang, Suki-
dc.contributor.alternativeNameShim, Hyo Sup-
dc.contributor.alternativeNameYoon, Joo Heon-
dc.contributor.alternativeNameCho, Nam Hoon-
dc.contributor.affiliatedAuthorKang, Suki-
dc.contributor.affiliatedAuthorShim, Hyo Sup-
dc.contributor.affiliatedAuthorYoon, Joo Heon-
dc.contributor.affiliatedAuthorCho, Nam Hoon-
dc.citation.volume9-
dc.citation.number2-
dc.citation.startPage1157-
dc.citation.endPage1164-
dc.identifier.bibliographicCitationJOURNAL OF PROTEOME RESEARCH, Vol.9(2) : 1157-1164, 2010-
dc.identifier.rimsid37823-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Otorhinolaryngology (이비인후과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers

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