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Molecular proteomics imaging of tumor interfaces by mass spectrometry

Authors
 Suki Kang  ;  Hyo Sup Shim  ;  Jong Sik Lee  ;  Dong Su Kim  ;  Hak Yong Kim  ;  Seong Hyun Hong  ;  Pan Soo Kim  ;  Joo Heon Yoon  ;  Nam Hoon Cho 
Citation
 JOURNAL OF PROTEOME RESEARCH, Vol.9(2) : 1157-1164, 2010 
Journal Title
JOURNAL OF PROTEOME RESEARCH
ISSN
 1535-3893 
Issue Date
2010
MeSH
Electrophoresis, Gel, Two-Dimensional ; Female ; Humans ; Immunohistochemistry ; Microscopy, Fluorescence ; Ovarian Neoplasms/chemistry* ; Principal Component Analysis ; Proteomics* ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods*
Keywords
2-dimensional gel electrophoresis ; interface ; MALDI-IMS ; ovarian cancer
Abstract
The specific molecular profiles of ovarian cancer interface zones (IZ), the region between tumors and normal tissues, were evaluated using a new method involving matrix-assisted laser desorption/ionization (MALDI)-imaging mass spectrometry (IMS). We analyzed three ovarian serous carcinomas using MALDI-IMS. Principal component analysis (PCA) was used to evaluate the quality of tissue spatial features based on MALDI-IMS, and for analysis of large data sets of MALDI-IMS. Two-dimensional gel electrophoresis and fluorescence microscopy were used to verify interface-specific proteins. Unique profiles were identified for the tumors, the normal zone, and the IZ. Through MALDI analysis, two interface-specific proteins, plastin 2 and peroxiredoxin 1 (PRDX 1), were identified as differentially regulated between zones. Fluorescence microscopy revealed high expression levels of plastin 2 and PRDX 1 along the IZ of ovarian tumors. This comparative proteomics study using tissue MALDI-IMS suggested that the IZ is different from the adjacent tumor and normal zones, and that plastin 2 and PRDX 1 may be interface markers specific to ovarian tumors
Full Text
http://pubs.acs.org/doi/abs/10.1021/pr900666q
DOI
10.1021/pr900666q
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Otorhinolaryngology (이비인후과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
Yonsei Authors
Kang, Suki(강숙희) ORCID logo https://orcid.org/0000-0002-9957-3479
Shim, Hyo Sup(심효섭) ORCID logo https://orcid.org/0000-0002-5718-3624
Yoon, Joo Heon(윤주헌)
Cho, Nam Hoon(조남훈) ORCID logo https://orcid.org/0000-0002-0045-6441
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/100789
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