0 290

Cited 50 times in

Dual priming oligonucleotide-based multiplex PCR analysis for detection of BRAFV600E mutation in FNAB samples of thyroid nodules in BRAFV600E mutation-prevalent area

Authors
 Jin Young Kwak  ;  Eun-Kyung Kim  ;  Jong-Kee Kim  ;  Jeong-Hyun Han  ;  Soon Won Hong  ;  Tae Sung Park  ;  Jong Rak Choi 
Citation
 HEAD AND NECK-JOURNAL FOR THE SCIENCES AND SPECIALTIES OF THE HEAD AND NECK, Vol.32(4) : 490-498, 2010 
Journal Title
 HEAD AND NECK-JOURNAL FOR THE SCIENCES AND SPECIALTIES OF THE HEAD AND NECK 
ISSN
 1043-3074 
Issue Date
2010
MeSH
Adult ; Aged ; Biopsy, Fine-Needle ; Carcinoma, Papillary/genetics ; Carcinoma, Papillary/pathology ; DNA, Neoplasm/genetics ; Female ; Humans ; Immunohistochemistry ; Male ; Middle Aged ; Mutation* ; Oligonucleotides/genetics ; Polymerase Chain Reaction ; Polymorphism, Restriction Fragment Length ; Proto-Oncogene Proteins B-raf/genetics* ; Proto-Oncogene Proteins B-raf/metabolism ; Risk Assessment ; Sensitivity and Specificity ; Thyroid Neoplasms/genetics* ; Thyroid Neoplasms/pathology* ; Thyroid Nodule/genetics* ; Thyroid Nodule/pathology*
Keywords
thyroid cancer ; BRAFV600Emutation ; direct DNAsequencing
Abstract
BACKGROUND: To evaluate the diagnostic value of dual priming oligonucleotide (DPO)-based multiplex polymerase chain reaction (PCR) for the detection of BRAF(V600E) mutations in ultrasound-guided fine-needle aspiration biopsy (US-FNAB) of thyroid nodules. METHODS: Our institutional review board approved this retrospective study, and informed consent was not required from patients. The 130 patients underwent US-FNAB to evaluate BRAF status in thyroid nodules. In FNAB washouts, DPO-based multiplex PCR, direct DNA sequencing, and PCR-restriction fragment length polymorphism (RFLP) were used to detect BRAF(V600E). The diagnostic performance of these methods was calculated. We compared cytologic results by BRAF status. RESULTS: Diagnostic accuracy and sensitivity were highest when screening with DPO-based multiplex PCR. BRAF(V600E) positivity was a useful marker at thyroid nodules with "suspicious for papillary thyroid carcinoma" or "inadequate" cytological result. CONCLUSIONS: DPO-based multiplex PCR may be an alternative to direct DNA sequencing because of its high sensitivity, high accuracy, and simplicity. BRAF(V600E) may be a useful additional diagnostic marker in BRAF(V600E)-prevalent areas.
Full Text
http://onlinelibrary.wiley.com/doi/10.1002/hed.21210/abstract
DOI
10.1002/hed.21210
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Radiology (영상의학교실) > 1. Journal Papers
Yonsei Authors
Kwak, Jin Young(곽진영) ORCID logo https://orcid.org/0000-0002-6212-1495
Kim, Eun-Kyung(김은경) ORCID logo https://orcid.org/0000-0002-3368-5013
Park, Tae Sung(박태성)
Choi, Jong Rak(최종락) ORCID logo https://orcid.org/0000-0002-0608-2989
Hong, Soon Won(홍순원) ORCID logo https://orcid.org/0000-0002-0324-2414
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/100733
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse