Cited 16 times in
DHP-derivative and low oxygen tension effectively induces human adipose stromal cell reprogramming
DC Field | Value | Language |
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dc.contributor.author | 김동욱 | - |
dc.date.accessioned | 2015-04-23T16:28:49Z | - |
dc.date.available | 2015-04-23T16:28:49Z | - |
dc.date.issued | 2010 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/100659 | - |
dc.description.abstract | BACKGROUND AND METHODS: In this study, we utilized a combination of low oxygen tension and a novel anti-oxidant, 4-(3,4-dihydroxy-phenyl)-derivative (DHP-d) to directly induce adipose tissue stromal cells (ATSC) to de-differentiate into more primitive stem cells. De-differentiated ATSCs was overexpress stemness genes, Rex-1, Oct-4, Sox-2, and Nanog. Additionally, demethylation of the regulatory regions of Rex-1, stemnesses, and HIF1alpha and scavenging of reactive oxygen species were finally resulted in an improved stem cell behavior of de-differentiate ATSC (de-ATSC). Proliferation activity of ATSCs after dedifferentiation was induced by REX1, Oct4, and JAK/STAT3 directly or indirectly. De-ATSCs showed increased migration activity that mediated by P38/JUNK and ERK phosphorylation. Moreover, regenerative efficacy of de-ATSC engrafted spinal cord-injured rats and chemical-induced diabetes animals were significantly restored their functions. CONCLUSIONS/SIGNIFICANCE: Our stem cell remodeling system may provide a good model which would provide insight into the molecular mechanisms underlying ATSC proliferation and transdifferentiation. Also, these multipotent stem cells can be harvested may provide us with a valuable reservoir of primitive and autologous stem cells for use in a broad spectrum of regenerative cell-based disease therapy | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | e9026 | - |
dc.relation.isPartOf | PLOS ONE | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Adipose Tissue/cytology | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Blotting, Western | - |
dc.subject.MESH | Catechols/pharmacology* | - |
dc.subject.MESH | Cell Dedifferentiation/drug effects* | - |
dc.subject.MESH | Cell Proliferation/drug effects | - |
dc.subject.MESH | Cell Transdifferentiation/drug effects | - |
dc.subject.MESH | Cell Transplantation/methods | - |
dc.subject.MESH | Cells, Cultured | - |
dc.subject.MESH | Diabetes Mellitus, Experimental/surgery | - |
dc.subject.MESH | Enzyme Activation/drug effects | - |
dc.subject.MESH | Female | - |
dc.subject.MESH | Gene Expression Profiling | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Mice, Inbred C57BL | - |
dc.subject.MESH | Mitogen-Activated Protein Kinases/metabolism | - |
dc.subject.MESH | Oxygen/pharmacology* | - |
dc.subject.MESH | Plant Extracts | - |
dc.subject.MESH | Polysaccharides/pharmacology* | - |
dc.subject.MESH | Rats | - |
dc.subject.MESH | Rats, Wistar | - |
dc.subject.MESH | Reverse Transcriptase Polymerase Chain Reaction | - |
dc.subject.MESH | Spinal Cord Injuries/surgery | - |
dc.subject.MESH | Stromal Cells/cytology* | - |
dc.subject.MESH | Stromal Cells/metabolism | - |
dc.subject.MESH | Stromal Cells/transplantation | - |
dc.title | DHP-derivative and low oxygen tension effectively induces human adipose stromal cell reprogramming | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Physiology (생리학) | - |
dc.contributor.googleauthor | Hyung Shin Yoon | - |
dc.contributor.googleauthor | Wha Young Kim | - |
dc.contributor.googleauthor | Jeong-Hoon Kim | - |
dc.identifier.doi | 10.1371/journal.pone.0009026 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00406 | - |
dc.relation.journalcode | J02540 | - |
dc.identifier.eissn | 1932-6203 | - |
dc.identifier.pmid | 20161735 | - |
dc.contributor.alternativeName | Kim, Dong Wook | - |
dc.contributor.affiliatedAuthor | Kim, Dong Wook | - |
dc.citation.volume | 5 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | e9026 | - |
dc.identifier.bibliographicCitation | PLOS ONE, Vol.5(2) : e9026, 2010 | - |
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