Cited 12 times in
Cardioprotection via modulation of calcium homeostasis by thiopental in hypoxia-reoxygenated neonatal rat cardiomyocytes
DC Field | Value | Language |
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dc.contributor.author | 황기철 | - |
dc.contributor.author | 박윤곤 | - |
dc.date.accessioned | 2015-04-23T16:27:17Z | - |
dc.date.available | 2015-04-23T16:27:17Z | - |
dc.date.issued | 2010 | - |
dc.identifier.issn | 0513-5796 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/100612 | - |
dc.description.abstract | PURPOSE: Ca(2+) homeostasis plays an important role in myocardial cell injury induced by hypoxia-reoxygenation, and prevention of intracellular Ca(2+) overload is key to cardioprotection. Even though thiopental is a frequently used anesthetic agent, little is known about its cardioprotective effects, particularly in association with Ca(2+) homeostasis. We investigated whether thiopental protects cardiomyocytes against hypoxia-reoxygenation injury by regulating Ca(2+) homeostasis. MATERIALS AND METHODS: Neonatal rat cardiomyocytes were isolated. Cardiomyocytes were exposed to different concentrations of thiopental and immediately replaced in the hypoxic chamber to maintain hypoxia. After 1 hour of exposure, a culture dish was transferred to the CO(2) incubator and cells were incubated at 37 for 5 hours. At the end of the experiments, the authors assessed cell protection using immunoblot analysis and caspase activity. The mRNA of genes involved in Ca(2+) homeostasis, mitochondrial membrane potential, and cellular Ca(2+) levels were examined. RESULTS: In thiopental-treated cardiomyocytes, there was a decrease in expression of the proapoptotic protein Bax, caspase-3 activation, and intracellular Ca(2+) content. In addition, both enhancement of anti-apoptotic protein Bcl-2 and activation of Erk concerned with survival were shown. Furthermore, thiopental attenuated alterations of genes involving Ca(2+) regulation and significantly modulated abnormal changes of NCX and SERCA2a genes in hypoxia-reoxygenated neonatal cardiomyocytes. Thiopental suppressed disruption of mitochondrial membrane potential (DeltaPsi(m)) induced by hypoxia-reoxygenation. CONCLUSION: Thiopental is likely to modulate expression of genes that regulate Ca(2+) homeostasis, which reduces apoptotic cell death and results in cardioprotection | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 187~196 | - |
dc.relation.isPartOf | YONSEI MEDICAL JOURNAL | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Apoptosis | - |
dc.subject.MESH | Calcium/metabolism* | - |
dc.subject.MESH | Cell Hypoxia/physiology* | - |
dc.subject.MESH | Cell Survival/drug effects | - |
dc.subject.MESH | Cells, Cultured | - |
dc.subject.MESH | GABA Modulators/pharmacology* | - |
dc.subject.MESH | Homeostasis/drug effects | - |
dc.subject.MESH | Immunoblotting | - |
dc.subject.MESH | In Situ Nick-End Labeling | - |
dc.subject.MESH | Membrane Potential, Mitochondrial/drug effects | - |
dc.subject.MESH | Microscopy, Confocal | - |
dc.subject.MESH | Myocytes, Cardiac/drug effects* | - |
dc.subject.MESH | Myocytes, Cardiac/metabolism* | - |
dc.subject.MESH | Rats | - |
dc.subject.MESH | Rats, Sprague-Dawley | - |
dc.subject.MESH | Reverse Transcriptase Polymerase Chain Reaction | - |
dc.subject.MESH | Thiopental/pharmacology* | - |
dc.title | Cardioprotection via modulation of calcium homeostasis by thiopental in hypoxia-reoxygenated neonatal rat cardiomyocytes | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Anesthesiology (마취통증의학) | - |
dc.contributor.googleauthor | Hyun-Soo Kim | - |
dc.contributor.googleauthor | Ki-Chul Hwang | - |
dc.contributor.googleauthor | Wyun-Kon Park | - |
dc.identifier.doi | 10.3349/ymj.2010.51.2.187 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A04456 | - |
dc.contributor.localId | A01593 | - |
dc.relation.journalcode | J02813 | - |
dc.identifier.eissn | 1976-2437 | - |
dc.identifier.pmid | 20191008 | - |
dc.subject.keyword | Ca2+ homeostasis | - |
dc.subject.keyword | Thiopental | - |
dc.subject.keyword | cardiomyocytes | - |
dc.subject.keyword | hypoxia-reoxygenation | - |
dc.contributor.alternativeName | Hwang, Ki Chul | - |
dc.contributor.alternativeName | Park, Wyun Kon | - |
dc.contributor.affiliatedAuthor | Hwang, Ki Chul | - |
dc.contributor.affiliatedAuthor | Park, Wyun Kon | - |
dc.citation.volume | 51 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 187 | - |
dc.citation.endPage | 196 | - |
dc.identifier.bibliographicCitation | YONSEI MEDICAL JOURNAL, Vol.51(2) : 187-196, 2010 | - |
dc.identifier.rimsid | 36603 | - |
dc.type.rims | ART | - |
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