Cited 3 times in
A dual-reporter system for specific tracing of pancreatic ss-cell lines that non-invasively measures viable in vivo islet cells
DC Field | Value | Language |
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dc.contributor.author | 김주영 | - |
dc.contributor.author | 이병완 | - |
dc.contributor.author | 이은직 | - |
dc.contributor.author | 이현철 | - |
dc.date.accessioned | 2015-04-23T16:22:44Z | - |
dc.date.available | 2015-04-23T16:22:44Z | - |
dc.date.issued | 2010 | - |
dc.identifier.issn | 0141-5492 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/100474 | - |
dc.description.abstract | Islet transplantation is a potential treatment for type 1 diabetes. Currently, islet graft survival is measured using invasive methods to determine blood glucose, insulin, and C-peptide levels, even though these variables have limited value. To trace beta-cell survival and functional status, we constructed an adenovirus/adenoassociate virus hybrid vector (Hyb-DR) carrying two reporter genes, luciferase and green fluorescent protein (GFP), linked by the internal ribosome entry site and driven by the rat insulin II promoter. Luciferase activity increased and positive GFP expression was observed in beta-cell lines (MIN6N8 and INS-1E) infected with Hyb-DR. Using an in vivo imaging instrument, the GFP signal was detected in the flanks of nude mice 2 weeks after transplanting Hyb-DR-infected MIN6 cells into the kidney capsule. Coinfection of Hyb-DR with plasmids carrying beta-cell-specific transcription factors also resulted in expression of luciferase and GFP in the non-beta-cell lines (HepG2, FL83B, and YGIC5). Thus, the dual reporter system provided quantitative and visual information about the functionality of the islet mass and activation of the insulin gene. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 53~57 | - |
dc.relation.isPartOf | BIOTECHNOLOGY LETTERS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Adenoviridae/genetics | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Cell Line | - |
dc.subject.MESH | Cell Line, Tumor | - |
dc.subject.MESH | Dependovirus/genetics | - |
dc.subject.MESH | Genetic Vectors/genetics | - |
dc.subject.MESH | Green Fluorescent Proteins/genetics | - |
dc.subject.MESH | Green Fluorescent Proteins/metabolism | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Insulin-Secreting Cells/cytology* | - |
dc.subject.MESH | Insulin-Secreting Cells/metabolism | - |
dc.subject.MESH | Insulin-Secreting Cells/transplantation* | - |
dc.subject.MESH | Islets of Langerhans/cytology* | - |
dc.subject.MESH | Islets of Langerhans/metabolism | - |
dc.subject.MESH | Luciferases/genetics | - |
dc.subject.MESH | Luciferases/metabolism | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Mice, Nude | - |
dc.subject.MESH | Rats | - |
dc.title | A dual-reporter system for specific tracing of pancreatic ss-cell lines that non-invasively measures viable in vivo islet cells | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Internal Medicine (내과학) | - |
dc.contributor.googleauthor | Suk Kyoung Kim | - |
dc.contributor.googleauthor | Joo Young Kim | - |
dc.contributor.googleauthor | Young Suk Choi | - |
dc.contributor.googleauthor | Mi-Kyung Lee | - |
dc.contributor.googleauthor | Byung Wan Lee | - |
dc.contributor.googleauthor | Hyun Chul Lee | - |
dc.contributor.googleauthor | Eun Jig Lee | - |
dc.identifier.doi | 10.1007/s10529-009-0113-3 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A02796 | - |
dc.contributor.localId | A03050 | - |
dc.contributor.localId | A03301 | - |
dc.contributor.localId | A00938 | - |
dc.relation.journalcode | J00336 | - |
dc.identifier.eissn | 1573-6776 | - |
dc.identifier.pmid | 19728108 | - |
dc.identifier.url | http://link.springer.com/article/10.1007%2Fs10529-009-0113-3 | - |
dc.subject.keyword | Adenovirus/adenoassociate virus hybrid vector | - |
dc.subject.keyword | Dual reporter | - |
dc.subject.keyword | GFP | - |
dc.subject.keyword | Insulin-producing cell | - |
dc.subject.keyword | Luciferase | - |
dc.contributor.alternativeName | Kim, Joo Young | - |
dc.contributor.alternativeName | Lee, Byung Wan | - |
dc.contributor.alternativeName | Lee, Eun Jig | - |
dc.contributor.alternativeName | Lee, Hyun Chul | - |
dc.contributor.affiliatedAuthor | Lee, Byung Wan | - |
dc.contributor.affiliatedAuthor | Lee, Eun Jig | - |
dc.contributor.affiliatedAuthor | Lee, Hyun Chul | - |
dc.contributor.affiliatedAuthor | Kim, Joo Young | - |
dc.citation.volume | 32 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 53 | - |
dc.citation.endPage | 57 | - |
dc.identifier.bibliographicCitation | BIOTECHNOLOGY LETTERS, Vol.32(1) : 53-57, 2010 | - |
dc.identifier.rimsid | 36508 | - |
dc.type.rims | ART | - |
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