0 289

Cited 18 times in

Capacitance-based real time monitoring of receptor-mediated endocytosis

Authors
 Rimi Lee  ;  Pyung Hwan Kim  ;  Joung-Woo Choi  ;  Kwon Oh-Joon  ;  Kyujung Kim  ;  Donghyun Kim  ;  Chae-Ok Yun  ;  Kyung-Hwa Yoo 
Citation
 BIOSENSORS & BIOELECTRONICS, Vol.25(6) : 1325-1332, 2010 
Journal Title
 BIOSENSORS & BIOELECTRONICS 
ISSN
 0956-5663 
Issue Date
2010
MeSH
Biosensing Techniques/instrumentation* ; Computer Systems ; Electric Capacitance ; Electrodes* ; Endocytosis/physiology* ; Equipment Design ; Equipment Failure Analysis ; Monitoring, Physiologic/instrumentation* ; Plethysmography, Impedance/instrumentation* ; Receptors, Cell Surface/physiology* ; Reproducibility of Results ; Sensitivity and Specificity
Abstract
Receptor-mediated endocytosis is essential for targeted gene/drug delivery to a specific cell type. In this study, we developed a capacitance sensor to monitor receptor-mediated endocytosis in real time. The capacitance sensor was able to detect a capacitance peak in different cell lines during the internalization of adenoviruses or antibodies via receptor-mediated endocytosis. In contrast, the capacitance declined without a capacitance peak when nanoparticles were taken up via non-specific pinocytosis. Thus, our capacitance sensor represents a potential capacitance-based means of discrimination between receptor-mediated endocytosis and non-specific pinocytosis. Moreover, we developed a capacitance sensor array to demonstrate capacitance-based high-throughput screening. We showed that the capacitance sensor array could rapidly identify antibodies or ligands with high specificity for target molecules. We propose that the capacitance sensor array will provide a valuable tool for high-throughput screening.
Full Text
http://www.sciencedirect.com/science/article/pii/S0956566309005703
DOI
10.1016/j.bios.2009.10.025
Appears in Collections:
1. College of Medicine (의과대학) > Medical Research Center (임상의학연구센터) > 1. Journal Papers
5. Research Institutes (연구소) > Institute for Cancer Research (암연구소) > 1. Journal Papers
Yonsei Authors
Kwon, Oh Joon(권오준)
Kim, Pyung Hwan(김평환)
Yun, Chae Ok(윤채옥)
Choi, Joung Woo(최정우)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/100450
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse