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Local application of periodontal ligament stromal cells promotes soft tissue regeneration

Authors
 H-S Baik  ;  J Park  ;  K-J Lee  ;  C Chung 
Citation
 ORAL DISEASES, Vol.20(6) : 574-581, 2014 
Journal Title
ORAL DISEASES
ISSN
 1354-523X 
Issue Date
2014
MeSH
Adolescent ; Animals ; Cell Movement/drug effects ; Cell Proliferation/drug effects ; Cells, Cultured ; Culture Media, Conditioned/chemistry ; Culture Media, Conditioned/pharmacology ; Female ; Fibroblasts ; Humans ; Keratinocytes ; Mice ; Periodontal Ligament/cytology* ; Regeneration* ; Skin Physiological Phenomena* ; Stromal Cells/transplantation ; Transforming Growth Factor beta1/analysis ; Young Adult
Keywords
periodontal ligament ; secretory factor ; soft tissue regeneration ; stem cell ; wound healing
Abstract
OBJECTIVES:
To test the potential stimulatory effect of local application of periodontal ligament (PDL) stromal cells on soft tissue regeneration.
MATERIALS AND METHODS:
Fluorescently labeled PDL cells outgrown from extracted human premolars or phosphate-buffered saline were locally injected to the cutaneous wounds created on mice. Soft tissue regeneration was evaluated for 14 days using photographs and histomorphometry. PDL cell engraftment was tracked with confocal microscopy. To detect the paracrine effect of the PDL cells on soft tissue regeneration, PDL cell-conditioned medium (CM) was evaluated for the concentration of secretory factors, transforming growth factor-beta 1 (TGFβ1). The effect of PDL CM on the proliferation and migration of dermal fibroblast and keratinocyte was tested using MTT assay and migration assay.
RESULTS:
The application of PDL cells significantly promoted soft tissue regeneration compared with the application of PBS. Self-replicating PDL cells were engrafted into the hair follicles of the host tissue. Dermal fibroblast proliferation and keratinocyte migration were significantly enhanced by the treatment with PDL CM. Physiologically significant amount of TGFβ1 was secreted from PDL cells into the CM.
CONCLUSION:
Local injection of PDL cells promoted soft tissue regeneration in part by the enhancement of fibroblast proliferation and keratinocyte migration through a paracrine mechanism.
Full Text
http://onlinelibrary.wiley.com/doi/10.1111/odi.12175/abstract
DOI
10.1111/odi.12175
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Orthodontics (교정과학교실) > 1. Journal Papers
Yonsei Authors
Baik, Hyoung Seon(백형선)
Lee, Kee Joon(이기준) ORCID logo https://orcid.org/0000-0002-0782-3128
Chung, Chooryung J.(정주령) ORCID logo https://orcid.org/0000-0001-9399-7193
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/100032
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