https://ir.ymlib.yonsei.ac.kr/handle/22282913/168976 Fri, 15 May 2026 08:11:13 GMT 2026-05-15T08:11:13Z https://ir.ymlib.yonsei.ac.kr/handle/22282913/211604 Title: On-site detection of airborne foodborne pathogens using a field-deployable recombinase polymerase amplification and CRISPR/Cas12a cleavage activity assay Authors: Jeong, Yeonwoo; Lee, Jina; Choi, Sangsoo; Shin, Dongmin; Jang, Soojin; Son, Seong Uk; Kang, Taejoon; Jung, Juyeon; Hwang, Jungho; Lim, Eun-Kyung Abstract: With the global increase in single-person households, the demand for meal kits is increasing, leading to the development of large-scale food production systems and complex supply chains. However, under the influence of global warming, these systems can be susceptible to food contamination, particularly by airborne foodborne bacteria. Conventional methods for detecting airborne bacteria involve complex, time-consuming, and laborintensive processes, which limit their applicability for field use and rapid food hygiene surveillance. In the present study, we developed a field-deployable diagnostic platform by combining recombinase polymerase amplification with CRISPR/Cas12a cleaVage Activity (RCCVA assay) for the rapid and sensitive identification of airborne foodborne bacteria. Airborne bacteria were collected using a self-developed electrostatic air sampler and analyzed using a portable isothermal amplification device. The RCCVA assay was designed to detect four major foodborne pathogens: Staphylococcus aureus, Salmonella enteritidis, Listeria monocytogenes, and Bacillus cereus. The limit of detection was measured as 274.9, 4.5, 9.5, and 28.5 culture-forming units (CFU)/mL, respectively, within 45 min. This platform enables early on-site detection of airborne pathogens within approximately 1 h (for the analytical phase) and shows potential for real-time monitoring in food processing environments, thereby contributing to improved public health and food safety. Wed, 01 Jul 2026 00:00:00 GMT https://ir.ymlib.yonsei.ac.kr/handle/22282913/211604 2026-07-01T00:00:00Z https://ir.ymlib.yonsei.ac.kr/handle/22282913/211413 Title: PLAY+ (Play, Land, Animals, You and +) framework: The role of active outdoor play in advancing One Health Authors: Lee, Eun-Young; Kim, Yeong-Bae; Cheng, Joy; Carbone, Mia; Abu-Omar, Karim; Bisung, Elijah; Ding, Ding; Hallal, Pedro C.; Jeon, Justin Y.; Kangmennaang, Joseph; Klopp, Jacquline M.; Lam, Steven; de Lannoy, Louise; Manyanga, Taru; Morrison, Shawnda A.; Silva, Diego Augusto Santos; Spence, John C.; Wachira, Lucy-Joy; Yi, Kyoung June; Tremblay, Mark S. Abstract: There has been growing recognition of the interconnections between human health, environmental sustainability, and ecosystem resilience. Positioning active outdoor play as a potential catalyst and ally in advancing human, animal, and environmental health, this study developed and validated a framework linking active outdoor play and One Health. The framework development followed a structured, multi-step process incorporating a scoping review, conceptual mapping, and expert consultations. Based on the results of the scoping review, a conceptual mapping exercise was undertaken to visually represent the interconnections between active outdoor play and One Health, which was then validated by key informants (n = 16) selected based on their expertise and leadership in the relevant topic areas. A total of 23 scholarly articles addressed the potential interconnectedness of active outdoor play, human-animal relationships, and environmental responsibility. Three themes emerged: 1) outdoor play environments and climate resilience (n = 6), 2) human-animal interactions and public health (n = 10), and 3) environmental stewardship and responsibility toward planetary health (n = 7). Nine policy documents were identified, of which eight did not make direct references to active outdoor play. Informed by the evidence, the PLAY+ (Play, Land, Animals, You and +) framework was created. Insights from an expert survey further refined the framework. While the framework highlights the interconnected benefits of active outdoor play across human, animal and environmental health domains, it is equally important to recognize associated risks and unintended consequences. As such, the framework should be views as conceptual, heuristic tool that encourages precautionary approaches, continued empricial evaluation, and context-sensitive implementation. Mon, 01 Jun 2026 00:00:00 GMT https://ir.ymlib.yonsei.ac.kr/handle/22282913/211413 2026-06-01T00:00:00Z https://ir.ymlib.yonsei.ac.kr/handle/22282913/211820 Title: TNFR Pathway-Related Proteins and Recurrent Coronary Artery Disease Events Authors: Lee, Jiwoo; Bellomo, Tiffany R.; Halford, Jennifer L.; Cho, So Mi Jemma; Koyama, Satoshi; Dron, Jacqueline; Haidermota, Sara; Ruan, Yunfeng; Urbut, Sarah; Truong, Buu; Patel, Aniruddh; Natarajan, Pradeep Abstract: BACKGROUND Despite optimization with lifestyle modifications and medications, complications of coronary artery disease (CAD) remain the leading cause of adult mortality worldwide. OBJECTIVES This study aimed to identify proteins and pathways linked to recurrent CAD events to better understand residual risk. METHODS We used data from 1,009 participants in the UK Biobank with baseline Olink plasma proteomic measures and CAD. Cox proportional hazards regression modeled the association between proteins measured and recurrent CAD events in follow-up. RESULTS Participants had a mean age of 62.51 years (SD 5.94) at enrollment; 183 (18.14%) were females and 656 (65.01%) had recurrent CAD events over 11.40 (IQR: 8.00-14.69) years of follow-up. Among 1,463 proteins tested, 102 proteins were independently associated with recurrent CAD events. Molecular functions were significantly enriched for tumor necrosis factor receptor (TNFR) activity by 100-fold (P = 6.37 & times; 10-10). Of the 16 proteins related to TNF annotated by the Gene Ontology database, tumor necrosis factor-alpha had a risk estimate of 1.36 (95% CI: 1.17-1.57; P = 6.38 & times; 10-5), TNFR1 (TNFRSF1A) had a risk estimate of 1.73 (95% CI: 1.43-2.09; P = 1.23 & times; 10-8), and TNFR2 (TNFRSF1B) had a risk estimate of 1.27 (95% CI: 1.13-1.44; P = 9.15 & times; 10-5) for recurrent CAD events. CONCLUSIONS Although TNFR1 and TNFR2 were initially thought to have opposing roles in cardiac remodeling postmyocardial infarction, this study highlights the complex interaction between these pathways and the need to identify specific inflammation-related targets to therapeutic strategies. (JACC Adv. 2026;5:102687) (c) 2026 The Authors. Published by Elsevier on behalf of the American College of Cardiology Foundation. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). Wed, 01 Apr 2026 00:00:00 GMT https://ir.ymlib.yonsei.ac.kr/handle/22282913/211820 2026-04-01T00:00:00Z https://ir.ymlib.yonsei.ac.kr/handle/22282913/211926 Title: A Cheonjiin Layout Mental Speller: Developing a Simple and Cost-Effective EEG-Based Brain-Computer Interface System Authors: Ahn, Ji Won; Yu, Gi Yeon; Kim, Seong-Wan; Seok, Young-Seek; Byun, Kyung-Min; Choi, Seung Ho Abstract: A brain-computer interface (BCI) enables direct communication between the brain and external devices by translating neural activity into executable control commands. Among electroencephalography (EEG)-based paradigms, steady-state visual evoked potential (SSVEP) is widely adopted due to its high signal-to-noise ratio, robustness, and minimal calibration requirements. While SSVEP-based spellers have been extensively investigated, many existing systems rely on high-channel-density EEG recordings and computationally complex processing pipelines, and are primarily designed for alphabetic input structures. In this study, we present an SSVEP-based Korean speller that integrates the Cheonjiin keyboard layout to support intuitive composition of Hangul syllables. The proposed system adopts a simple configuration, employing only five visual stimulation frequencies (6.67-12 Hz) and two occipital EEG channels (O1 and O2), with real-time frequency recognition performed using canonical correlation analysis (CCA) within a 1.5 s sliding window. EEG signals were acquired at 200 Hz using an OpenBCI Ganglion board, band-pass filtered (5-45 Hz), and processed with harmonic sinusoidal reference templates for multi-frequency classification. The proposed interface generates five control commands (up, down, left, right, and select), enabling directional cursor navigation and character confirmation on a 4 & times; 4 virtual Cheonjiin keyboard. Experimental validation with three healthy participants demonstrated an average classification accuracy of approximately 82% and an information transfer rate (ITR) of 31.2 bits/min. Frequency-domain analysis revealed clear spectral peaks at the stimulation frequencies and their harmonics, indicating reliable SSVEP responses. The proposed system employs a simple two-channel configuration integrated with a Korean language-specific input structure, demonstrating that reliable SSVEP-based communication can be realized without computationally intensive algorithms or high-cost EEG acquisition systems. These findings demonstrate that reliable SSVEP-based communication can be achieved using a low-channel configuration without reliance on high-cost EEG equipment. Wed, 01 Apr 2026 00:00:00 GMT https://ir.ymlib.yonsei.ac.kr/handle/22282913/211926 2026-04-01T00:00:00Z