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On-site detection of airborne foodborne pathogens using a field-deployable recombinase polymerase amplification and CRISPR/Cas12a cleavage activity assay

Wed, 01 Jul 2026 00:00:00 GMT

Title: On-site detection of airborne foodborne pathogens using a field-deployable recombinase polymerase amplification and CRISPR/Cas12a cleavage activity assay Authors: Jeong, Yeonwoo; Lee, Jina; Choi, Sangsoo; Shin, Dongmin; Jang, Soojin; Son, Seong Uk; Kang, Taejoon; Jung, Juyeon; Hwang, Jungho; Lim, Eun-Kyung Abstract: With the global increase in single-person households, the demand for meal kits is increasing, leading to the development of large-scale food production systems and complex supply chains. However, under the influence of global warming, these systems can be susceptible to food contamination, particularly by airborne foodborne bacteria. Conventional methods for detecting airborne bacteria involve complex, time-consuming, and laborintensive processes, which limit their applicability for field use and rapid food hygiene surveillance. In the present study, we developed a field-deployable diagnostic platform by combining recombinase polymerase amplification with CRISPR/Cas12a cleaVage Activity (RCCVA assay) for the rapid and sensitive identification of airborne foodborne bacteria. Airborne bacteria were collected using a self-developed electrostatic air sampler and analyzed using a portable isothermal amplification device. The RCCVA assay was designed to detect four major foodborne pathogens: Staphylococcus aureus, Salmonella enteritidis, Listeria monocytogenes, and Bacillus cereus. The limit of detection was measured as 274.9, 4.5, 9.5, and 28.5 culture-forming units (CFU)/mL, respectively, within 45 min. This platform enables early on-site detection of airborne pathogens within approximately 1 h (for the analytical phase) and shows potential for real-time monitoring in food processing environments, thereby contributing to improved public health and food safety.

PLAY+ (Play, Land, Animals, You and +) framework: The role of active outdoor play in advancing One Health

Mon, 01 Jun 2026 00:00:00 GMT

Title: PLAY+ (Play, Land, Animals, You and +) framework: The role of active outdoor play in advancing One Health Authors: Lee, Eun-Young; Kim, Yeong-Bae; Cheng, Joy; Carbone, Mia; Abu-Omar, Karim; Bisung, Elijah; Ding, Ding; Hallal, Pedro C.; Jeon, Justin Y.; Kangmennaang, Joseph; Klopp, Jacquline M.; Lam, Steven; de Lannoy, Louise; Manyanga, Taru; Morrison, Shawnda A.; Silva, Diego Augusto Santos; Spence, John C.; Wachira, Lucy-Joy; Yi, Kyoung June; Tremblay, Mark S. Abstract: There has been growing recognition of the interconnections between human health, environmental sustainability, and ecosystem resilience. Positioning active outdoor play as a potential catalyst and ally in advancing human, animal, and environmental health, this study developed and validated a framework linking active outdoor play and One Health. The framework development followed a structured, multi-step process incorporating a scoping review, conceptual mapping, and expert consultations. Based on the results of the scoping review, a conceptual mapping exercise was undertaken to visually represent the interconnections between active outdoor play and One Health, which was then validated by key informants (n = 16) selected based on their expertise and leadership in the relevant topic areas. A total of 23 scholarly articles addressed the potential interconnectedness of active outdoor play, human-animal relationships, and environmental responsibility. Three themes emerged: 1) outdoor play environments and climate resilience (n = 6), 2) human-animal interactions and public health (n = 10), and 3) environmental stewardship and responsibility toward planetary health (n = 7). Nine policy documents were identified, of which eight did not make direct references to active outdoor play. Informed by the evidence, the PLAY+ (Play, Land, Animals, You and +) framework was created. Insights from an expert survey further refined the framework. While the framework highlights the interconnected benefits of active outdoor play across human, animal and environmental health domains, it is equally important to recognize associated risks and unintended consequences. As such, the framework should be views as conceptual, heuristic tool that encourages precautionary approaches, continued empricial evaluation, and context-sensitive implementation.

TNFR Pathway-Related Proteins and Recurrent Coronary Artery Disease Events

Wed, 01 Apr 2026 00:00:00 GMT

Title: TNFR Pathway-Related Proteins and Recurrent Coronary Artery Disease Events Authors: Lee, Jiwoo; Bellomo, Tiffany R.; Halford, Jennifer L.; Cho, So Mi Jemma; Koyama, Satoshi; Dron, Jacqueline; Haidermota, Sara; Ruan, Yunfeng; Urbut, Sarah; Truong, Buu; Patel, Aniruddh; Natarajan, Pradeep Abstract: BACKGROUND Despite optimization with lifestyle modifications and medications, complications of coronary artery disease (CAD) remain the leading cause of adult mortality worldwide. OBJECTIVES This study aimed to identify proteins and pathways linked to recurrent CAD events to better understand residual risk. METHODS We used data from 1,009 participants in the UK Biobank with baseline Olink plasma proteomic measures and CAD. Cox proportional hazards regression modeled the association between proteins measured and recurrent CAD events in follow-up. RESULTS Participants had a mean age of 62.51 years (SD 5.94) at enrollment; 183 (18.14%) were females and 656 (65.01%) had recurrent CAD events over 11.40 (IQR: 8.00-14.69) years of follow-up. Among 1,463 proteins tested, 102 proteins were independently associated with recurrent CAD events. Molecular functions were significantly enriched for tumor necrosis factor receptor (TNFR) activity by 100-fold (P = 6.37 & times; 10-10). Of the 16 proteins related to TNF annotated by the Gene Ontology database, tumor necrosis factor-alpha had a risk estimate of 1.36 (95% CI: 1.17-1.57; P = 6.38 & times; 10-5), TNFR1 (TNFRSF1A) had a risk estimate of 1.73 (95% CI: 1.43-2.09; P = 1.23 & times; 10-8), and TNFR2 (TNFRSF1B) had a risk estimate of 1.27 (95% CI: 1.13-1.44; P = 9.15 & times; 10-5) for recurrent CAD events. CONCLUSIONS Although TNFR1 and TNFR2 were initially thought to have opposing roles in cardiac remodeling postmyocardial infarction, this study highlights the complex interaction between these pathways and the need to identify specific inflammation-related targets to therapeutic strategies. (JACC Adv. 2026;5:102687) (c) 2026 The Authors. Published by Elsevier on behalf of the American College of Cardiology Foundation. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

Blood Pressure Polygenic Score Predicts Long-Term Blood Pressure Control and Treatment-Resistant Hypertension

Sun, 01 Mar 2026 00:00:00 GMT

Title: Blood Pressure Polygenic Score Predicts Long-Term Blood Pressure Control and Treatment-Resistant Hypertension Authors: Cho, So Mi Jemma; Ruan, Yunfeng; Lee, Hyeok-Hee; Koyama, Satoshi; Juraschek, Stephen P.; Allen, Norrina B.; Yang, Eugene; Mcevoy, John W.; Secemsky, Eric A.; Honigberg, Michael C.; Fahed, Akl C.; Patel, Aniruddh P.; Hornsby, Whitney E.; Natarajan, Pradeep Abstract: BACKGROUND: Suboptimal blood pressure (BP) control remains a major cardiovascular disease risk factor. Whether genetically predicted BP independently predicts long-term BP control is unknown. We examined the associations of BP polygenic scores (PGSs) with long-term BP control and treatment-resistant hypertension. METHODS: We identified 22 456 Mass General Brigham Biobank participants with hypertension. Longitudinal BP control was defined as the percentage of time above-target systolic BP (SBP) >= 130 mm Hg or diastolic BP (DBP) >= 80 mm Hg over 5 years. Using multivariable regression, we assessed the associations of BP PGS with duration above-target BP and lifetime treatment-resistant hypertension incidence. Incremental prognostic utility of BP PGSs was assessed based on the discrimination C-index, Brier score, and net reclassification index. Validation was performed in the population-based UK Biobank cohort using the SBP/DBP >= 140/90 mm Hg threshold. RESULTS: Among 10 853 (48.3%) were female, the mean SBP/DBP (SD) at index date was 132 (18)/75 (11) mm Hg, and 4126 (18.4%) developed treatment-resistant hypertension over lifetime. In reference to the low (<20th percentile) PGS group, the high (>= 80th percentile) BP PGS was associated with 8.01 (95% CI, 6.68%-9.34%) longer duration with above-target SBP and 6.19 (95% CI, 5.05%-7.33%) with high DBP. Each high SBP and DBP PGS conferred 2.36 (95% CI, 2.07-2.68) and 1.75 (95% CI, 1.55-1.99)-fold higher odds of treatment-resistant hypertension. Adding BP PGSs to traditional risk factors improved treatment-resistant hypertension prediction from C-index (95% CI), 0.74 (0.73-0.75) to 0.78 (0.77-0.79). BP PGSs consistently predicted longitudinal BP management to a comparable extent in the UK Biobank. CONCLUSIONS: Harnessing BP PGSs may inform anticipated trends in BP control to warrant vigilant monitoring and augment prioritization of intensive therapy.