https://ir.ymlib.yonsei.ac.kr/handle/22282913/168748 Mon, 20 Apr 2026 08:49:34 GMT 2026-04-20T08:49:34Z https://ir.ymlib.yonsei.ac.kr/handle/22282913/211654 Title: Review of the Historical and Technical Development of DNA Based Single-molecule Force Sensors Authors: Vellampatti, Srivithya; Truong, Tham; Tran, Phuong; Le, Tram Thi Hong; Kim, Byoung Choul; 김병철 Abstract: Mechanobiology has evolved from macroscopic anatomical studies to a precise molecular understanding of how cells sense and respond to physical forces. While conventional tools like atomic force microscopy and traction force microscopy established the field, they often face trade-offs between force sensitivity and high-throughput spatial mapping in living cells. This review explores the transformative rise of DNA-based single-molecule force sensors, which leverage the programmability and defined physical attributes of nucleic acids to bridge this gap. We critically analyze the design principles and mechanical characteristics of four primary sensor classes: DNA duplexes, DNA hairpins, DNA origami, and DNA G-quadruplexes. Special attention is given to their fabrication and tuning for detecting piconewton-scale events. Furthermore, we highlight versatile applications ranging from dissecting cellular mechanotransduction to high-resolution bioimaging and biosensing. Finally, we discuss recent advances, projecting a future era of "mechano-medicine" where active DNA nanodevices profile mechanical phenotypes for diagnostic and therapeutic innovation. Sun, 01 Mar 2026 00:00:00 GMT https://ir.ymlib.yonsei.ac.kr/handle/22282913/211654 2026-03-01T00:00:00Z https://ir.ymlib.yonsei.ac.kr/handle/22282913/211559 Title: Single Cell-Seeded Human Intestinal Organoids for Organoid Research Authors: Sim, Young; Kim, Ha Young; Jung, Gil Young; Axelrad, Jordan E.; Cadwell, Ken; Jang, Kyung Ku Abstract: Recent developments in organoid technology have enabled the creation of patient-derived intestinal organoids (PDIOs) that recapitulate the structural, functional, genetic, and epigenetic features of their original tissues. However, conventional passage-derived organoids inevitably yield heterogeneous populations in size and number, leading to inconsistent results even under identical conditions. To address this, a standardized approach, referred to here as "single cell-seeded PDIOs,"was established. In this method, mature PDIOs were enzymatically dissociated into single cells and seeded at a defined number into individual wells of a 96-well plate. This controlled seeding normalized the size and number of PDIOs. Compared with passage-derived organoids, single cell-seeded PDIOs displayed reduced inter-well variability in organoid numbers and intra-well variability in organoid sizes, which enables the determination of generation efficiency and improves the reproducibility of viability assays. Moreover, this platform is compatible with downstream analysis, including transcriptomic analysis and protein expression profiling. Collectively, this approach may enhance experimental consistency and provide a practical foundation for reproducible PDIO-based studies. Sun, 01 Feb 2026 00:00:00 GMT https://ir.ymlib.yonsei.ac.kr/handle/22282913/211559 2026-02-01T00:00:00Z https://ir.ymlib.yonsei.ac.kr/handle/22282913/211592 Title: Microplastic-Induced Macrophage Dysfunction Drives Lung Tumor Progression through Glutathione Imbalance Authors: Kim, Bora; Park, Koung-Min; Lee, Haerang; Hyun, Young-Min; 김보라 Abstract: Microplastics (MPs) are emerging contaminants whose immunological consequences remain poorly defined. Here, we investigated MP-induced immune responses using bone marrow-derived macrophages and a lung tumor model to delineate how MPs modulate tumor immunity. MPs triggered TLR2- and TLR4-dependent signaling pathways in macrophages, which initiated AP-1 signaling and lysosomal destabilization, followed by mitochondrial depolarization and excessive reactive oxygen species production. Despite NRF2 pathway activation, GPX1 and GPX3 were selectively suppressed, revealing a paradoxical uncoupling of glutathione metabolism that precipitated macrophage ferroptosis. In vivo, orally ingested MPs accumulated across multiple organs. In the lungs of tumor-bearing mice, MP exposure led to a time-dependent remodeling of the immune microenvironment, characterized by marked infiltration of M1-like macrophages and functional impairment of lymphocytes at later stages, which was accompanied by increased tumor burden. These findings identify an immune-redox-ferroptosis axis driven by glutathione imbalance and suggest redox disruption as a mechanistic link between microplastic exposure and tumor progression. Sun, 01 Feb 2026 00:00:00 GMT https://ir.ymlib.yonsei.ac.kr/handle/22282913/211592 2026-02-01T00:00:00Z https://ir.ymlib.yonsei.ac.kr/handle/22282913/211142 Title: Beneficial effects of SGLT1/2 and SGLT2 inhibitors on vaso-occlusive events and organ damage in sickle cell disease mice Authors: Jin, Bo-Ram; Kumari, Tripti; Lee, Jingu; Kim, Jae-Sung; Bokorova, Radka; Gheware, Atish; Ripoll, Carla Valenzuela; Sargazi, Alireza; Jeong, Soi; Hyun, Young-Min; Rehman, Sana Saif Ur; Razani, Babak; Lee, Janet S.; Javaheri, Ali; Cho, Jaehyung Abstract: Aims Sodium-glucose co-transporter 2 inhibitors are widely used to treat patients with type 2 diabetes and exhibit beneficial cardiovascular effects beyond glucose lowering. In this study, we investigated their potential to alleviate vaso-occlusive events and organ damage in sickle cell disease (SCD) mice.Methods and results Intravital and immunofluorescence microscopy reveal that a 4-day oral administration of dapagliflozin (DAPA) or sotagliflozin (SOTA) significantly reduces neutrophil adhesion and transmigration in cremaster venules, with SOTA showing greater inhibition, and down-regulates E-selectin and intercellular adhesion molecule-1 (ICAM-1) expression in cremaster venules of TNF-alpha-challenged SCD mice. Intriguingly, only SOTA improves mouse survival acutely. Similar inhibitory effects on neutrophil recruitment are observed in SCD mice subjected to hypoxia-reoxygenation. Flow chamber assays indicate that neither drug directly affects neutrophil or endothelial cell adhesive function. In addition, treatment of neutrophils and platelets from SCD mice and patients with DAPA or SOTA does not affect their activation. When administered for 4 months, DAPA or SOTA mitigates neutrophil recruitment and enhances microcirculation in cremaster venules of TNF-alpha-challenged SCD mice, while only SOTA confers a survival benefit. Both drugs reduce leucocyte infiltration in the liver or lungs, suggesting their ability to protect against organ damage. Co-administration with hydroxyurea for 4 months does not enhance these effects. Multiplex analysis shows that DAPA and SOTA lower plasma levels of soluble P-selectin, ICAM-1, S100A8/A9, and pro-inflammatory cytokines in SCD mice.Conclusion Our findings suggest that DAPA and SOTA mitigate vaso-occlusive events in SCD, with SOTA providing superior benefits. Thu, 01 Jan 2026 00:00:00 GMT https://ir.ymlib.yonsei.ac.kr/handle/22282913/211142 2026-01-01T00:00:00Z