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Microfluidic pulp platform with vascular integration to evaluate biofunctional material

2026-06-09T06:14:46Z

Title: Microfluidic pulp platform with vascular integration to evaluate biofunctional material Authors: Lee, Min-Yong; Mangal, Utkarsh; Yoon, Hi-Won; Im, Hyungsoon; Choi, Sung-Hwan; Kwon, Jae Sung; Shin, Su-Jung Abstract: Biofunctional materials are increasingly used to preserve tooth vitality by promoting dental pulp-mediated hard tissue formation. However, existing evaluation platforms, such as conventional in vitro assays or microfluidic systems, fail to replicate the complex histological and physiological characteristics of dental pulp. This study introduces a 4D biofunctional material-to-pulp (4D BFP) platform that recapitulates pulp physiology, integrating three key features of native pulp tissue: layered histoarchitecture, microcirculatory dynamics, and threedimensional multicellular organization. This platform further incorporates a temporal dimension by simulating age-dependent vascular transitions, thereby enabling the age-specific modelling of pulp responses, and defining the system as a 4D microfluidic pulp model. Computational fluid dynamics confirmed physiologically relevant flow profiles, while the compartmentalized design supported the spatially organized co-culture of endothelial cell (EC) and human dental pulp stem cell (hDPSC) spheroids. Functional responses to biofunctional material were assessed in both young and mature 4D pulp models. Transcriptomic profiling revealed distinct age-and material-specific signatures related to cellular growth arrest, angiogenesis, and developmental pathways. Collectively, the 4D BFP platform provides a physiological and temporal biomimetic model to study biomaterial-dental pulp interactions, supporting its application as a primary screening tool for candidate biofunctional materials.

Elevated C-reactive Protein Levels and Malignant Transformation Risk in Patients With Oral Mucosal Diseases

2026-06-09T06:14:47Z

Title: Elevated C-reactive Protein Levels and Malignant Transformation Risk in Patients With Oral Mucosal Diseases Authors: Kang, Jeong-Hyun Abstract: Introduction and aims: Chronic inflammation is recognised as a hallmark of cancers. This multicentre cohort study investigated the influence of elevated serum C-reactive protein (CRP) levels on the malignant transformation risk of oral inflammatory diseases and lesions with malignant potential utilising the Observational Medical Outcomes Partnership Common Data Model. Methods: This retrospective, observational cohort study included the data from 9 medical centres. CRP levels recorded within 30 days of oral diseases diagnosis were analysed. A meta-regression identified 0.269 mg/dL as the optimal threshold for predicting malignancy. Patients were classified into elevated CRP (CRP >= 0.269 mg/dL) and control (CRP <0.269 mg/dL) groups. Cox proportional hazard models were applied to estimate risk of developing oral squamous cell carcinoma (OSCC), followed by 1:1 and 1:2 propensity score matching and pooled meta-analyses. Results: In total, 10029 individuals with oral diseases and available CRP data were included, comprising 4142 and 5887 in the elevated CRP and control groups, respectively. Overall, 204 individuals (2.03%) progressed to OSCC, with higher incidence in the elevated CRP group (2.32%) compared with controls (1.83%). Elevated CRP was significantly associated with increased OSCC risk in both 1:1 (HR = 1.79 [95% CI 1.08-2.95]), and 1:2 propensity score matching models (HR = 1.55 [95% CI 1.01-2.38]). Conclusions: Elevated CRP levels, indicative of systemic inflammation, were linked to an increased risk of malignant progression in patients with inflammatory oral conditions and lesions with malignant potential. Understanding the role of inflammation may help assess OSCC risk and improve prevention. (c) 2026 The Authors. Published by Elsevier Inc. on behalf of FDI World Dental Federation. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)

In Vitro Shear Bond Strength of Additively Manufactured Denture Base Resins to Hard Chairside Reline Materials

2026-06-11T06:44:49Z

Title: In Vitro Shear Bond Strength of Additively Manufactured Denture Base Resins to Hard Chairside Reline Materials Authors: Yoo, Je-Hyeon; Kim, Jimin; Park, Yeseul; Kim, Jee-Hwan Abstract: Introduction and aims: Reliable bonding between additively manufactured denture base resins and hard chairside reline materials is essential for long-term clinical performance, yet evidence under simulated aging conditions remains limited. This in vitro study evaluated the shear bond strength (SBS) of two hard chairside reline materials to conventional polymethyl methacrylate (PMMA) and two additively manufactured denture base resins under thermocycling conditions simulating relining at denture delivery and after clinical service. Methods: One heat-polymerized PMMA resin and two additively manufactured denture base resins composed of urethane dimethacrylate (UDMA) and methacrylate ester monomer (MA) were bonded to Tokuyama Rebase II (R) or Ufi Gel Hard (U), forming six material combinations. Specimens were assigned to three conditions: non-thermocycling (NT), thermocycling before relining (TB), or thermocycling after relining (TA). Ten specimens were prepared per subgroup (n = 180). Specimens that exhibited complete debonding during thermocycling were assigned an SBS value of 0 MPa and included in the analysis. SBS was measured using a universal testing machine, and failure modes were classified. Data were analysed using appropriate statistical tests (alpha = 0.05). Results: SBS was significantly influenced by denture base resin type, reline material, and thermocycling condition (P < .05). U demonstrated higher SBS than R across all denture base resins. Under NT conditions, the MA-U group showed the highest SBS (6.78 +/- 1.57 MPa), and the UDMA-R group showed the lowest (1.41 +/- 0.49 MPa). After thermocycling, 80% of UDMA-R and 20% of MA-R specimens debonded, whereas no failures occurred in the U groups. R predominantly exhibited adhesive failures, while U mainly showed cohesive or mixed failures. Conclusion: Within the limitations of this in vitro study, bonding performance between denture base resins and hard chairside reline materials was influenced by material compatibility and thermal aging. U demonstrated greater resistance to thermally induced debonding than R.

Antimicrobial DMAHDM nanoparticle-modified resin for 3D printing: Composition-Property relationships

2026-06-12T07:45:47Z

Title: Antimicrobial DMAHDM nanoparticle-modified resin for 3D printing: Composition-Property relationships Authors: Jin, Gan; Liu, Yunqi; Lee, Dae Gyun; Shim, Min Suk; Cho, Seok Hwan; Lee, Du-Hyeong; Kim, Jong-Eun Abstract: This study aimed to determine the effects of the proportion of dimethylaminohexadecyl methacrylate (DMAHDM) nanoparticle to urethane acrylate (UA)-based 3D-printing resin on antibacterial and antifungal activities, biocompatibility, degree of conversion (DC), and mechanical properties. UA-based resin and DMAHDM were synthesized separately and mixed to prepare specimens at DMAHDM proportions of 0-1 wt%. The WST-8 viability assay was used to assess the antibacterial effects against Streptococcus mutans (S. mutans), and the growth of Candida albicans (C. albicans) was measured by OD600 to evaluate antifungal activity. Human gingival fibroblasts were used for biocompatibility assessment via WST-8 and EdU assay. The DC was analyzed using FTIR spectroscopy. Flexural strength, flexural modulus, and Vickers hardness were assessed. One-way ANOVA was performed (alpha = 0.05). The anti-S. mutans efficacy increased with the DMAHDM concentration, with the bacterial viability significantly decreasing from 85.76 f 14.78% (mean f standard deviation) at 0 wt% to 56.61 f 0.60% at 1 wt%. C. albicans growth was also inhibited in a dose-dependent manner, decreasing from 88.77 f 5.03% at 0 wt% to 44.00 f 14.72% at 1 wt%. No cytotoxicity was observed but the cell proliferation decreased as the DMAHDM concentration increased. The highest DC was recorded at 1 wt% (57.88 f 0.83%). The flexural strength was largest (118.59 f 19.89 MPa) at 0 wt%. The Vickers hardness was highest at 0 wt% (23.03 f 0.76 HV) and decreased slightly with increasing DMAHDM concentration. DMAHDM-incorporated UAbased 3D-printing resin exhibited strong antibacterial effects against S. mutans and antifungal effects against C. albicans, alongside excellent biocompatibility, improved polymer conversion, and competitive mechanical properties, highlighting its potential for dental applications.