Cited 3 times in
Extent of Mycobacterium bovis infection in dairy cattle herds subject to partial culling as determined by an interferon-gamma assay
DC Field | Value | Language |
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dc.contributor.author | 조상래 | - |
dc.date.accessioned | 2015-01-06T17:26:35Z | - |
dc.date.available | 2015-01-06T17:26:35Z | - |
dc.date.issued | 2014 | - |
dc.identifier.issn | 1229-845X | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/99988 | - |
dc.description.abstract | The interferon-gamma (IFN-γ) assay is employed as a complementary diagnostic test for bovine tuberculosis (BTB) in many countries. To simplify this assay, we established a 96-well plate format using the ESAT-6 and CFP-10 antigens and then employed it to determine the extent of Mycobacterium (M.) bovis infection in dairy herds with a history of BTB outbreaks in a country where only selective culling is practiced. The sensitivity and specificity of this IFN-γ assay were 85.9% and 100%, respectively, based on comparison with the conventional single intradermal tuberculin test (SIDT). The IFN-γ assay was also positive in 30.4% and 36.8% of SIDT-negative animals from herds with recent and remote BTB outbreaks, respectively. Of 14 SIDT-negative, IFN-γ positive cattle, five (35.7%) were culture positive and an additional six were positive based on a polymerase chain reaction-based test for M. bovis. Therefore, the IFN-γ assay has the potential to serve as a specific and sensitive test for M. bovis infection in dairy cattle. Further, the results indicated that a substantial portion of SIDT-negative animals in herds with previous BTB outbreaks were actually infected with M. bovis. Accordingly, the present selective-culling strategy may require modifications to include this more sensitive assay. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 259~265 | - |
dc.relation.isPartOf | JOURNAL OF VETERINARY SCIENCE | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | Extent of Mycobacterium bovis infection in dairy cattle herds subject to partial culling as determined by an interferon-gamma assay | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Microbiology (미생물학) | - |
dc.contributor.googleauthor | Sungmo Je | - |
dc.contributor.googleauthor | Un-chang Yeo | - |
dc.contributor.googleauthor | Taeksun Song | - |
dc.contributor.googleauthor | Ki-cheol Kim | - |
dc.contributor.googleauthor | Sung-Yun Park | - |
dc.contributor.googleauthor | Man-Jung Kim | - |
dc.contributor.googleauthor | Sang-Nae Cho | - |
dc.identifier.doi | 10.4142/jvs.2014.15.2.259 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A03824 | - |
dc.relation.journalcode | J01927 | - |
dc.identifier.eissn | 1976-555X | - |
dc.subject.keyword | bovine tuberculosis | - |
dc.subject.keyword | IFN-gamma assay | - |
dc.subject.keyword | Mycobacterium bovis | - |
dc.contributor.alternativeName | Cho, Sang Nae | - |
dc.contributor.affiliatedAuthor | Cho, Sang Nae | - |
dc.citation.volume | 15 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 259 | - |
dc.citation.endPage | 265 | - |
dc.identifier.bibliographicCitation | JOURNAL OF VETERINARY SCIENCE, Vol.15(2) : 259-265, 2014 | - |
dc.identifier.rimsid | 54391 | - |
dc.type.rims | ART | - |
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