Cited 14 times in
Molecular recognition of protecolytic activity in metastatic cancer cells using fluorogenic gold nanoprobes
DC Field | Value | Language |
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dc.contributor.author | 구민희 | - |
dc.contributor.author | 서미란 | - |
dc.contributor.author | 서진석 | - |
dc.contributor.author | 양재문 | - |
dc.contributor.author | 육종인 | - |
dc.contributor.author | 이유진 | - |
dc.contributor.author | 이은직 | - |
dc.contributor.author | 허단 | - |
dc.contributor.author | 허용민 | - |
dc.contributor.author | 황승연 | - |
dc.contributor.author | 이현정 | - |
dc.date.accessioned | 2015-01-06T16:48:27Z | - |
dc.date.available | 2015-01-06T16:48:27Z | - |
dc.date.issued | 2014 | - |
dc.identifier.issn | 0956-5663 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/98807 | - |
dc.description.abstract | We describe the development of biomarker-sensitive nanoprobes based on nanoparticle surface energy transfer (NSET) effect that enabling recognition of the expression of membrane type-1 matrix metalloproteinase (MT1-MMP) anchored on invasive cancer cells and its proteolytic activity simultaneously. First of all, we confirmed invasiveness of cancer cell lines (HT1080 and MCF7) via migration and invasion assay. We also prepared gold nanoparticle (GNP) acts as a quencher for fluorescein isothiocyanate (FITC). This FITC is conjugated in end-terminal of activatable fluorogenic peptide (ActFP). The ActFP attach to surface of GNP (GNP-ActFP) for a targeting moiety and proteolytic activity ligand toward MT1-MMP. The GNP-ActFP can generate fluorescence signal when ActFP is cleaved by proteolytic activity after targeting toward MT1-MMP. In order to study specificity for MT1-MMP, GNP-ActFP is treated to HT1080 and MCF7 cells, and then, we determine the in vitro targeting potential and fluorogenic activity of GNP-ActFP for MT1-MMP via fluorescence multi-reader. We also confirmed fluorogenic activity of GNP-ActFP via confocal microscopic imaging, and finally, endocytosis of GNP-ActFP is observed via cellular transmission electron microscopic imaging. | - |
dc.description.statementOfResponsibility | open | - |
dc.relation.isPartOf | BIOSENSORS & BIOELECTRONICS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Biosensing Techniques/methods* | - |
dc.subject.MESH | Cell Line, Tumor | - |
dc.subject.MESH | Cell Movement | - |
dc.subject.MESH | Fluorescence | - |
dc.subject.MESH | Fluorescent Dyes*/chemistry | - |
dc.subject.MESH | Gold*/chemistry | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | MCF-7 Cells | - |
dc.subject.MESH | Matrix Metalloproteinase 14/analysis* | - |
dc.subject.MESH | Matrix Metalloproteinase 14/metabolism | - |
dc.subject.MESH | Nanoparticles*/chemistry | - |
dc.subject.MESH | Nanoparticles*/ultrastructure | - |
dc.subject.MESH | Neoplasm Metastasis/diagnosis* | - |
dc.subject.MESH | Neoplasm Metastasis/pathology | - |
dc.subject.MESH | Neoplasms/diagnosis | - |
dc.subject.MESH | Neoplasms/enzymology | - |
dc.subject.MESH | Neoplasms/pathology* | - |
dc.subject.MESH | Optical Imaging/methods | - |
dc.subject.MESH | Proteolysis | - |
dc.title | Molecular recognition of protecolytic activity in metastatic cancer cells using fluorogenic gold nanoprobes | - |
dc.type | Article | - |
dc.contributor.college | Researcher Institutes (부설 연구소) | - |
dc.contributor.department | Yonsei Integrative Research Institute for Cerebral & Cardiovascular Disease (뇌심혈관질환융합연구사업단) | - |
dc.contributor.googleauthor | Yoochan Hong | - |
dc.contributor.googleauthor | Minhee Ku | - |
dc.contributor.googleauthor | Dan Heo | - |
dc.contributor.googleauthor | Seungyeon Hwang | - |
dc.contributor.googleauthor | Eugene Lee | - |
dc.contributor.googleauthor | Joseph Park | - |
dc.contributor.googleauthor | Jihye Choi | - |
dc.contributor.googleauthor | Hyeon Jung Lee | - |
dc.contributor.googleauthor | Miran Seo | - |
dc.contributor.googleauthor | Eun Jig Lee | - |
dc.contributor.googleauthor | Jong In Yook | - |
dc.contributor.googleauthor | Seungjoo Haam | - |
dc.contributor.googleauthor | Yong-Min Huh | - |
dc.contributor.googleauthor | Dae Sung Yoon | - |
dc.contributor.googleauthor | Jin-Suck Suh | - |
dc.contributor.googleauthor | Jaemoon Yang | - |
dc.identifier.doi | 10.1016/j.bios.2014.02.011 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00191 | - |
dc.contributor.localId | A01876 | - |
dc.contributor.localId | A01916 | - |
dc.contributor.localId | A02536 | - |
dc.contributor.localId | A03016 | - |
dc.contributor.localId | A03050 | - |
dc.contributor.localId | A04346 | - |
dc.contributor.localId | A04359 | - |
dc.contributor.localId | A04469 | - |
dc.contributor.localId | A02315 | - |
dc.relation.journalcode | J00330 | - |
dc.identifier.eissn | 1873-4235 | - |
dc.identifier.pmid | 24583688 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S095656631400089X | - |
dc.subject.keyword | Cancer metastasis | - |
dc.subject.keyword | Fluorescence | - |
dc.subject.keyword | Membrane type 1-matrix metalloproteinase (MT1-MMP) | - |
dc.subject.keyword | Nanoparticle surface energy transfer (NSET) | - |
dc.subject.keyword | Nanoprobe | - |
dc.subject.keyword | Proteolysis | - |
dc.contributor.alternativeName | Ku, Min Hee | - |
dc.contributor.alternativeName | Seo, Mi Ran | - |
dc.contributor.alternativeName | Suh, Jin Suck | - |
dc.contributor.alternativeName | Yang, Jae Moon | - |
dc.contributor.alternativeName | Yook, Jong In | - |
dc.contributor.alternativeName | Lee, Eu Gene | - |
dc.contributor.alternativeName | Lee, Eun Jig | - |
dc.contributor.alternativeName | Heo, Dan | - |
dc.contributor.alternativeName | Huh, Yong Min | - |
dc.contributor.alternativeName | Hwang, Seung Yeon | - |
dc.contributor.affiliatedAuthor | Ku, Min Hee | - |
dc.contributor.affiliatedAuthor | Seo, Mi Ran | - |
dc.contributor.affiliatedAuthor | Suh, Jin Suck | - |
dc.contributor.affiliatedAuthor | Yook, Jong In | - |
dc.contributor.affiliatedAuthor | Lee, Eu Gene | - |
dc.contributor.affiliatedAuthor | Lee, Eun Jig | - |
dc.contributor.affiliatedAuthor | Heo, Dan | - |
dc.contributor.affiliatedAuthor | Huh, Yong Min | - |
dc.contributor.affiliatedAuthor | Hwang, Seung Yeon | - |
dc.contributor.affiliatedAuthor | Yang, Jae Moon | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 15 | - |
dc.citation.number | 57 | - |
dc.citation.startPage | 171 | - |
dc.citation.endPage | 178 | - |
dc.identifier.bibliographicCitation | BIOSENSORS & BIOELECTRONICS, Vol.15(57) : 171-178, 2014 | - |
dc.identifier.rimsid | 39257 | - |
dc.type.rims | ART | - |
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