Cited 82 times in
Cholesterol level of lipid raft microdomains regulates apoptotic cell death in prostate cancer cells through EGFR-mediated Akt and ERK signal transduction
DC Field | Value | Language |
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dc.contributor.author | 정병하 | - |
dc.contributor.author | 조강수 | - |
dc.contributor.author | 홍성준 | - |
dc.date.accessioned | 2014-12-21T17:17:49Z | - |
dc.date.available | 2014-12-21T17:17:49Z | - |
dc.date.issued | 2007 | - |
dc.identifier.issn | 0270-4137 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/97322 | - |
dc.description.abstract | BACKGROUND: Lipid rafts are cholesterol-enriched microdomains in cell membranes that have been shown to regulate signal transduction. We investigated whether membrane cholesterol could regulate apoptosis and attempted to elucidate the mechanism by which apoptosis is induced in prostate cancer cells. METHODS: LNCaP cells were exposed to 2-hydroxyprophyl-beta-cyclodextrin (HPCD) to deplete membrane cholesterol. Cell viability and apoptosis were evaluated by Celltiter Bluetrade mark Cell Viability assay and ethidium bromide/acridine orange staining. Signal transduction was investigated by immunoblot analysis of cell lysates. RESULTS: Cell viability was dose dependent inhibited by HPCD and restored by replenishment of cholesterol. HPCD induced apoptotic cell death through down-regulation of Bcl-xL and up-regulation of caspase-3 and PARP cleavages. HPCD inhibited both EGFR/Akt and EGFR/ERK signal transduction. CONCLUSIONS: Lipid raft cholesterol regulates apoptotic cell death in prostate cancer cells through EGFR-mediated Akt and ERK pathways. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 1061~1069 | - |
dc.relation.isPartOf | PROSTATE | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | Cholesterol level of lipid raft microdomains regulates apoptotic cell death in prostate cancer cells through EGFR-mediated Akt and ERK signal transduction | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Urology (비뇨기과학) | - |
dc.contributor.googleauthor | Hea Young Oh | - |
dc.contributor.googleauthor | Eun Jin Lee | - |
dc.contributor.googleauthor | Sung Joon Hong | - |
dc.contributor.googleauthor | Kang Su Cho | - |
dc.contributor.googleauthor | Byung Ha Chung | - |
dc.contributor.googleauthor | Sun Yoon | - |
dc.identifier.doi | 10.1002/pros.20593 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A03607 | - |
dc.contributor.localId | A03801 | - |
dc.contributor.localId | A04402 | - |
dc.relation.journalcode | J02557 | - |
dc.identifier.eissn | 1097-0045 | - |
dc.identifier.url | http://onlinelibrary.wiley.com/doi/10.1002/pros.20593/abstract | - |
dc.contributor.alternativeName | Chung, Byung Ha | - |
dc.contributor.alternativeName | Cho, Kang Su | - |
dc.contributor.alternativeName | Hong, Sung Joon | - |
dc.contributor.affiliatedAuthor | Chung, Byung Ha | - |
dc.contributor.affiliatedAuthor | Cho, Kang Su | - |
dc.contributor.affiliatedAuthor | Hong, Sung Joon | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 67 | - |
dc.citation.number | 10 | - |
dc.citation.startPage | 1061 | - |
dc.citation.endPage | 1069 | - |
dc.identifier.bibliographicCitation | PROSTATE, Vol.67(10) : 1061-1069, 2007 | - |
dc.identifier.rimsid | 50439 | - |
dc.type.rims | ART | - |
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