Cited 19 times in
Upstream stimulatory factors regulate the C/EBPα gene during differentiation of 3T3-L1 preadipocytes
DC Field | Value | Language |
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dc.contributor.author | 김재우 | - |
dc.date.accessioned | 2014-12-21T16:26:09Z | - |
dc.date.available | 2014-12-21T16:26:09Z | - |
dc.date.issued | 2007 | - |
dc.identifier.issn | 0006-291X | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/95688 | - |
dc.description.abstract | During adipocyte differentiation, CCAAT/enhancer-binding protein α (C/EBPα) functions as a pleiotropic transcriptional activator of numerous adipocyte genes. The promoter of the C/EBPα gene has an E-box upstream of C/EBP binding site. Deletion or mutation of the E-box decreases promoter activity, suggesting that the E-box participates in the regulation of C/EBPα expression. Protein binding to the E-box during the adipocyte differentiation is increased as indicated by EMSA and UV cross-linking. Purification of the E-box binding proteins from differentiated 3T3-L1 adipocytes, showed that USF and AP-4 are associated with the E-box. Supershift analysis showed that USF1 and USF2 bind to this element as heterodimers, whereas the addition of anti-AP-4 antibody enhanced the binding complex, suggesting that AP-4 negatively regulates the promoter activity. The expression of AP-4 is reciprocally regulated with USF-1 during adipocyte differentiation. These findings suggest that USF-1 and 2 play roles in C/EBPα expression, whereas the AP-4 represses it. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 517~521 | - |
dc.relation.isPartOf | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | Upstream stimulatory factors regulate the C/EBPα gene during differentiation of 3T3-L1 preadipocytes | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Biochemistry & Molecular Biology (생화학,분자생물학) | - |
dc.contributor.googleauthor | Jae-woo Kim | - |
dc.contributor.googleauthor | Henrik Monila | - |
dc.contributor.googleauthor | M. Daniel Lane | - |
dc.contributor.googleauthor | Akhilesh Pandey | - |
dc.identifier.doi | 10.1016/j.bbrc.2007.01.008 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00865 | - |
dc.relation.journalcode | J00281 | - |
dc.identifier.eissn | 1090-2104 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S0006291X07000393 | - |
dc.contributor.alternativeName | Kim, Jae Woo | - |
dc.contributor.affiliatedAuthor | Kim, Jae Woo | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 354 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 517 | - |
dc.citation.endPage | 521 | - |
dc.identifier.bibliographicCitation | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Vol.354(2) : 517-521, 2007 | - |
dc.identifier.rimsid | 45023 | - |
dc.type.rims | ART | - |
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