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Fecal colonization of Enterobacteriaceae carrying plasmid-mediated quinolone resistance determinants in Korea

Authors
 Haeng Soon Jeong  ;  Il Kwon Bae  ;  Jeong Hwan Shin  ;  Si Hyun Kim  ;  Chulhun L. Chang  ;  Joseph Jeong  ;  Sunjoo Kim  ;  Chae Hoon Lee  ;  Nam Hee Ryoo  ;  Jeong Nyeo Lee 
Citation
 MICROBIAL DRUG RESISTANCE, Vol.17(4) : 507-512, 2011 
Journal Title
MICROBIAL DRUG RESISTANCE
ISSN
 1076-6294 
Issue Date
2011
MeSH
Anti-Bacterial Agents/pharmacology ; Bacterial Proteins/genetics ; Conjugation, Genetic ; Drug Resistance, Bacterial/genetics* ; Enterobacteriaceae/classification ; Enterobacteriaceae/drug effects ; Enterobacteriaceae/genetics ; Enterobacteriaceae/isolation & purification* ; Enterobacteriaceae Infections/epidemiology* ; Enterobacteriaceae Infections/microbiology ; Feces/microbiology* ; Hospitals, University ; Humans ; Microbial Sensitivity Tests ; Plasmids/genetics* ; Polymerase Chain Reaction ; Quinolones/pharmacology* ; Republic of Korea/epidemiology
Abstract
The aims of the current study were to investigate the prevalence and molecular characteristics of plasmid-mediated quinolone resistance (PMQR) genes from colonizing fecal organisms and to compare the incidence and subtype of these genes according to bacterial species and hospital at five tertiary-care hospitals in Korea. A total of 500 nonduplicated clinical isolates of Enterobacteriaceae were obtained from fecal specimens at five tertiary-care hospitals between March and May 2008. The PMQR genes (qnrA, qnrB, qnrS, aac(6')-Ib-cr, and qepA) were amplified by PCR and confirmed by direct sequencing of the PCR products. A total of 83 (16.6%) qnr-positive isolates were detected. The prevalence rates of qnrA, qnrB, and qnrS were 1.4%, 13.6%, and 1.6%, respectively. The species distributions of qnrB-positive isolates were Klebsiella pneumoniae (37/109; 33.9%), Citrobacter freundii (10/34; 29.4%), Citrobacter braakii (8/13; 61.5%), and Escherichia coli (8/275; 2.9%). Sixteen subtypes of qnrB were detected, including seven novel variants. The prevalences of aac(6')-Ib-cr and qepA were 15.6% (n=78) and 0.6% (n=3), respectively. The aac(6')-Ib-cr gene was detected in 39 (47.0%) of 83 qnr-positive isolates and 39 (9.4%) of 417 qnr-negative isolates There was one qepA variant containing a novel mutation (Ala231Val). The prevalence of PMQR genes was high in Enterobacteriaceae from stool specimens in Korea, and there was a close relation between qnr and aac(6')-Ib-cr.
Full Text
http://online.liebertpub.com/doi/abs/10.1089/mdr.2011.0040
DOI
10.1089/mdr.2011.0040
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
Bae, Il Kwon(배일권)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/95249
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