Development of DNA Sensor Using Magnetic Iron Oxide Nanoparticle
Journal of The Institute of Electronics Engineers of Korea (전자공학회논문지 - SC)
Journal of The Institute of Electronics Engineers of Korea (전자공학회논문지 - SC), Vol.48(6) : 51~56, 2011
The surface of magnetic iron oxide nanoparticles (γ-Fe₂O₃) is functionalized (-NH₂, -COOH) with bifunctional organic molecules and evaluated using FT-IR (Fourier transform infrared spectroscopy). We immobilize 21-base pair probe DNA and hybridize fluorescence-labeled (Cy5) target DNA onto the functionalized iron oxide nanoparticles. The fluorescence images obtained from a confocal microscopy show that the functionalized iron oxide nanoparticles should detect the hybridization of complementary and noncomplementary DNA.