Cited 5 times in
Multiple effects of the cellular prion protein on tooth development.
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김성오 | - |
dc.date.accessioned | 2014-12-20T17:39:52Z | - |
dc.date.available | 2014-12-20T17:39:52Z | - |
dc.date.issued | 2011 | - |
dc.identifier.issn | 0214-6282 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/95027 | - |
dc.description.abstract | The role of the prion protein (PrP) in transmissible spongiform encephalopathies has been the focus of intense investigation. However, less is known about the physiological function of normal cellular PrP (PrP(C)). In adult human teeth, PrP(C) has been identified in odontoblasts, cementoblasts and epithelial remnants of Malassez. In this study, we have localized PrP(C) in developing human and mouse teeth, and investigated the function of PrP using a PrP-knockout (Prnp(0/0) ) mouse model. PrP(C) was detected in developing human and mouse ameloblasts and odontoblasts. In vitro, undifferentiated dental mesenchymal cells from embryonic day 18 (E18) Prnp(0/0) mouse molars proliferated much more rapidly compared to age-matched, wild-type (wt) mouse molar dental mesenchymal cells. Histochemistry and immunohistochemical analyses showed a subtle but measurable phenotype, with the absence of PrP resulting in earlier initiation of both dentin and enamel formation. Consistent with this finding, laser microdissected odontoblasts from newborn Prnp(0/0) mouse incisors had a reduced proliferation rate, as measured by the expression of proliferating cell nuclear antigen (PCNA), and increased type 1 collagen mRNA expression. Dentin microhardness of the fully erupted molars was reduced and incisal enamel mineralization was delayed in Prnp(0/0) compared to age-matched wt mouse teeth. Taken together, these results suggest that PrP(C) affects multiple processes involved in tooth formation, through regulating the differentiation of ameloblasts and odontoblasts. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 953~960 | - |
dc.relation.isPartOf | INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Ameloblasts/cytology | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Collagen Type I/metabolism | - |
dc.subject.MESH | Gene Expression Regulation, Developmental* | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Immunohistochemistry/methods | - |
dc.subject.MESH | In Situ Hybridization | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Mice, Knockout | - |
dc.subject.MESH | Molar/embryology | - |
dc.subject.MESH | Odontoblasts/cytology | - |
dc.subject.MESH | Prions/metabolism* | - |
dc.subject.MESH | Proliferating Cell Nuclear Antigen/biosynthesis | - |
dc.subject.MESH | Time Factors | - |
dc.subject.MESH | Tooth/embryology* | - |
dc.title | Multiple effects of the cellular prion protein on tooth development. | - |
dc.type | Article | - |
dc.contributor.college | College of Dentistry (치과대학) | - |
dc.contributor.department | Dept. of Pediatric Dentistry (소아치과학) | - |
dc.contributor.googleauthor | Yan Zhang | - |
dc.contributor.googleauthor | Seong-Oh Kim | - |
dc.contributor.googleauthor | Sibylle Opsahl-Vital | - |
dc.contributor.googleauthor | Sunita P. Ho | - |
dc.contributor.googleauthor | Jean-Baptiste Souron | - |
dc.contributor.googleauthor | Charles Kim | - |
dc.contributor.googleauthor | Kurt Giles | - |
dc.contributor.googleauthor | Pamela K. Den Besten | - |
dc.identifier.doi | 10.1387/ijdb.113348yz | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00577 | - |
dc.relation.journalcode | J01106 | - |
dc.identifier.eissn | 1696-3547 | - |
dc.identifier.pmid | 22252492 | - |
dc.subject.keyword | tooth development | - |
dc.subject.keyword | prion protein | - |
dc.subject.keyword | ameloblast | - |
dc.subject.keyword | odontoblast | - |
dc.contributor.alternativeName | Kim, Seong Oh | - |
dc.contributor.affiliatedAuthor | Kim, Seong Oh | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 55 | - |
dc.citation.number | 10-12 | - |
dc.citation.startPage | 953 | - |
dc.citation.endPage | 960 | - |
dc.identifier.bibliographicCitation | INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY, Vol.55(10-12) : 953-960, 2011 | - |
dc.identifier.rimsid | 26991 | - |
dc.type.rims | ART | - |
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