An orthotopic nude mouse model of tongue carcinoma
Journal of the Korean Association of Oral and Maxillofacial Surgeons (대한구강악안면외과학회지 )
Journal of the Korean Association of Oral and Maxillofacial Surgeons (대한구강악안면외과학회지 ), Vol.37(6) : 490~495, 2011
Introduction: Development of carcinoma on oral tongue may cause bilateral cervical lymph node metastasis, rapid invasion and growth of the cancer
cells due to rich blood supply in muscle tissues. It is not only difficult to develop an animal experimental model, but also to proceed follow-up research after
the development of such model as the induction of cancer lead to difficulty in taking nutrition for the experimental animals that often causes early death.
Materials and Methods: IIn this study, author have transplanted YD-10Bmod cells into nude mouse oral tongues with different cells number (5×104,
5×105, 5×106 cells/mouse) and observed the development aspect of oral tongue cancers.
Results: The cancer developed from orthotopic transplantation of YD-10Bmod cells into nude mouse oral tongue show invasion and central necrosis
of the tumor, similar to the cancers developed human oral tongue cancer. The difference in tumor size and the time of central necrosis development
depending on the number of transplanted tumor cells shows the feasibility of extending the survival period of the nude mouse by limiting the
transplanted tumor cells to <5×104 cells/mouse or under per nude mouse.
Conclusion: This nude mouse model could be used effectively in developing effective chemotheray agent and establishing an animal experimental
model that can be used to study the mechanism of cervical lymph node metastasis of the oral tongue cancer.