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Effects of the immobilization of heparin and rhPDGF-BB to titanium surfaces for the enhancement of osteoblastic functions and anti-inflammation.

Authors
 Jung-Bo Huh ; Jeong-Yol Lee ; Kyung-Lae Lee ; Sung-Eun Kim ; Mi-Jung Yun ; Ji-Suk Shim ; June-Sung Shim ; Sang-Wan Shin 
Citation
 Journal of Advanced Prosthodontics, Vol.3(3) : 152~60, 2011 
Journal Title
 Journal of Advanced Prosthodontics 
ISSN
 2005-7806 
Issue Date
2011
Abstract
PURPOSE: This study was to investigate the effects of recombinant human platelet-derived growth factor (rhPDGF-BB) and heparin to titanium surfaces for enhancement of osteoblastic functions and inhibition of inflammation activity. MATERIALS AND METHODS: The anodized titanium discs, not coated with any material, were used as a control group. In heparinized- Ti group, dopamine was anchored to the surface of Ti substrates, and coated with heparin. In PDGF-Ti group, rhPDGF-BB was immobilized onto heparinized Ti surface. The surface morphologies were investigated by the scanning electron microscope in each group. The release kinetics of rhPDGF-BB were analyzed, and cytotoxicity tests for each group were conducted. The biocompatibilities were characterized by measuring cell proliferation, alkaline phosphatase activity, and calcium deposition using MG-63 cells. Statistical comparisons were carried out by one-way ANOVA tests. Differences were considered statistically significant at (*)P<.05 and (**)P<.001. RESULTS: The combination of rhPDGF-BB and heparin stimulated alkaline phosphatase activity and OCN mRNA expression in osteoblastic cells ((*)P<.05 and (**)P<.001). MG-63 cells grown on PDGF-Ti had significantly higher amounts of calcium deposition than those grown on anodized Ti ((**)P<.001). Heparinized Ti was more anti-inflammatory compared to anodized Ti, when exposed to lipopolysaccharide using the transcript levels of TNF-α and IL-6 of proinflammatory cytokine ((*)P<.05 and (**)P<.001). CONCLUSION: The result of this study demonstrated that the incorporation of rhPDGF-BB and heparin onto Ti surface enhanced osteoblastic functions and inhibited inflammation.
URI
http://ir.ymlib.yonsei.ac.kr/handle/22282913/94819
DOI
10.4047/jap.2011.3.3.152
Appears in Collections:
1. 연구논문 > 2. College of Dentistry > Dept. of Prosthodontics
Yonsei Authors
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