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Measurement of fluid secretion from intact airway submucosal glands

Authors
 Jeffrey J. Wine ; Nam Soo Joo ; Kim V. Tran ; Alan W. Cuthbert ; John W. Hanrahan ; Juan Ianowski ; Toshiya Irokawa ; Monal Khansaheb ; Jin V. Wu ; Mauri E. Krouse ; Hyung-Ju Cho ; Jae Young Choi 
Citation
 Methods in Molecular Biology , Vol.742 : 93~112, 2011 
Journal Title
 Methods in Molecular Biology 
ISSN
 1064-3745 
Issue Date
2011
Abstract
Human airways are kept sterile by a mucosal innate defense system that includes mucus secretion. Mucus is secreted in healthy upper airways primarily by submucosal glands and consists of defense molecules mixed with mucins, electrolytes, and water and is also a major component of sputum. Mucus traps pathogens and mechanically removes them via mucociliary clearance while inhibiting their growth via molecular (e.g., lysozyme) and cellular (e.g., neutrophils, macrophages) defenses. Fluid secretion rates of single glands in response to various mediators can be measured by trapping the primary gland mucus secretions in an oil layer, where they form spherical bubbles that can be optically measured at any desired interval to provide detailed temporal analysis of secretion rates. The composition and properties of the mucus (e.g., solids, viscosity, pH) can also be determined. These methods have now been applied to mice, ferrets, cats, pigs, sheep, and humans, with a main goal of comparing gland secretion in control and CFTR-deficient humans and animals
URI
http://ir.ymlib.yonsei.ac.kr/handle/22282913/94568
DOI
10.1007/978-1-61779-120-8_6
Appears in Collections:
1. 연구논문 > 1. College of Medicine > Dept. of Otorhinolaryngology
Yonsei Authors
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Link
 http://link.springer.com/protocol/10.1007%2F978-1-61779-120-8_6
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