Cited 0 times in

Genome-wide YFP fluorescence complementation screen identifies new regulators for telomere signaling in human cells

Authors
 Ok-Hee Lee ; Hyeung Kim ; Zhou Songyang ; Dan Liu ; Amin Safari ; Heekyung Kate Chae ; Jiancong Liang ; Liuh-Yow Chen ; Dong Yang ; Hwa Jin Baek ; Quanyuan He 
Citation
 Molecular & Cellular Proteomics, Vol.10(2) : M110.001628, 2011 
Journal Title
 Molecular & Cellular Proteomics 
ISSN
 1535-9476 
Issue Date
2011
Abstract
Detection of low-affinity or transient interactions can be a bottleneck in our understanding of signaling networks. To address this problem, we developed an arrayed screening strategy based on protein complementation to systematically investigate protein-protein interactions in live human cells, and performed a large-scale screen for regulators of telomeres. Maintenance of vertebrate telomeres requires the concerted action of members of the Telomere Interactome, built upon the six core telomeric proteins TRF1, TRF2, RAP1, TIN2, TPP1, and POT1. Of the ∼12,000 human proteins examined, we identified over 300 proteins that associated with the six core telomeric proteins. The majority of the identified proteins have not been previously linked to telomere biology, including regulators of post-translational modifications such as protein kinases and ubiquitin E3 ligases. Results from this study shed light on the molecular niche that is fundamental to telomere regulation in humans, and provide a valuable tool to investigate signaling pathways in mammalian cells.
URI
http://ir.ymlib.yonsei.ac.kr/handle/22282913/94495
DOI
10.1074/mcp.M110.001628–1
Appears in Collections:
1. 연구논문 > 5. Research Institutes > Yonsei Integrative Research Institute for Cerebral & Cardiovascular Disease
Yonsei Authors
사서에게 알리기
  feedback
Files in This Item:
T201103848.pdfDownload
Export
RIS (EndNote)
XLS (Excel)
XML

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse