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Calpains are involved in Entamoeba histolytica-induced death of HT-29 colonic epithelial cells

Title
Calpains are involved in Entamoeba histolytica-induced death of HT-29 colonic epithelial cells
Authors
Yun Soo Jang;Kyoung-Ju Song;Myeong Heon Shin;Sang Kyou Lee;Kyeong Ah Kim;Young Ah Lee;Ju Young Kim
Issue Date
2011
Journal Title
Korean Journal of Parasitology
ISSN
0023-4001
Citation
Korean Journal of Parasitology, Vol.49(2) : 177~180, 2011
Abstract
Entamoeba histolytica is an enteric tissue-invading protozoan parasite that can cause amebic colitis and liver abscess in humans. E. histolytica has the capability to kill colon epithelial cells in vitro; however, information regarding the role of calpain in colon cell death induced by ameba is limited. In this study, we investigated whether calpains are involved in the E. histolytica-induced cell death of HT-29 colonic epithelial cells. When HT-29 cells were co-incubated with E. histolytica, the propidium iodide stained dead cells markedly increased compared to that in HT-29 cells incubated with medium alone. This pro-death effect induced by ameba was effectively blocked by pretreatment of HT-29 cells with the calpain inhibitor, calpeptin. Moreover, knockdown of m- and µ-calpain by siRNA significantly reduced E. histolytica-induced HT-29 cell death. These results suggest that m- and µ-calpain may be involved in colon epithelial cell death induced by E. histolytica.
URI

http://ir.ymlib.yonsei.ac.kr/handle/22282913/94478
DOI
10.3347/kjp.2011.49.2.177
Appears in Collections:
1. 연구논문 > 1. College of Medicine > Dept. of Environmental Medical Biology
Yonsei Authors
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