Calpains are involved in Entamoeba histolytica-induced death of HT-29 colonic epithelial cells
Yun Soo Jang ; Kyoung-Ju Song ; Myeong Heon Shin ; Sang Kyou Lee ; Kyeong Ah Kim ; Young Ah Lee ; Ju Young Kim
Korean Journal of Parasitology, Vol.49(2) : 177~180, 2011
Korean Journal of Parasitology
Entamoeba histolytica is an enteric tissue-invading protozoan parasite that can cause amebic colitis and liver abscess in humans. E. histolytica has the capability to kill colon epithelial cells in vitro; however, information regarding the role of calpain in colon cell death induced by ameba is limited. In this study, we investigated whether calpains are involved in the E. histolytica-induced cell death of HT-29 colonic epithelial cells. When HT-29 cells were co-incubated with E. histolytica, the propidium iodide stained dead cells markedly increased compared to that in HT-29 cells incubated with medium alone. This pro-death effect induced by ameba was effectively blocked by pretreatment of HT-29 cells with the calpain inhibitor, calpeptin. Moreover, knockdown of m- and µ-calpain by siRNA significantly reduced E. histolytica-induced HT-29 cell death. These results suggest that m- and µ-calpain may be involved in colon epithelial cell death induced by E. histolytica.