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Altered epidermal lipid layers induced by long-term exposure to suberythemal-dose ultraviolet

Authors
 Hana Bak  ;  Seung-phil Hong  ;  Se-Kyoo Jeong  ;  Eung-Ho Choi  ;  Sang E. Lee  ;  Seung-Hun Lee  ;  Sung-Ku Ahn 
Citation
 INTERNATIONAL JOURNAL OF DERMATOLOGY, Vol.50(7) : 832-837, 2011 
Journal Title
INTERNATIONAL JOURNAL OF DERMATOLOGY
ISSN
 0011-9059 
Issue Date
2011
MeSH
Animals ; Ceramides/metabolism ; Cholesterol Esters/metabolism ; Dose-Response Relationship, Radiation ; Epidermis/metabolism ; Epidermis/pathology* ; Epidermis/radiation effects* ; Erythema/prevention & control ; Fatty Acid Synthases/genetics ; Female ; Gene Expression Regulation, Enzymologic/radiation effects ; Hydroxymethylglutaryl CoA Reductases/genetics ; Mice ; Mice, Hairless ; Receptors, Cell Surface/metabolism ; Serine C-Palmitoyltransferase/genetics ; Skin Aging/pathology ; Skin Aging/radiation effects* ; UltravioletRays/adverse effects* ; Water/metabolism
Abstract
BACKGROUND: Although several studies have reported on the biological effects of ultraviolet (UV) radiation, there have been only a few reports on the changes in epidermal lipids following long-term UV irradiation at suberythemal dose (SED), to which people are usually exposed during their lifetime.

OBJECTIVES: To investigate the changes of epidermal lipid properties after long-term UV radiation with SED.

MATERIALS AND METHODS: Hairless mice were irradiated three times weekly for 15 weeks at an SED of UV (UVB: 20 mJ/cm(2) ; UVA: 14 J/cm(2) ). Every three weeks, transepidermal water loss (TEWL) was measured by a Tewameter. The morphological alterations of stratum corneum (SC) lipid lamellae were examined by electron microscopy (EM). Activities of three key enzymes for mRNA of serine palmitoyl transferase, fatty acid synthase, and HMG CoA reductase were analyzed with real time reverse transcriptase-polymerase chain reaction. We also measured the amount of ceramide, cholesterol sulfate, and free fatty acid in the SC by high-performance thin-layer chromatography with exposed times.

RESULTS: The SED UV-irradiated group showed increased TEWL after 12 weeks. Following the irradiation period, EM revealed incomplete and separated lamellae at SC intercellular space. mRNA of three key enzymes was increased until six weeks of UV irradiation and decreased thereafter. However, three major lipid amounts gradually decreased throughout the exposed period, with a notable decrease in ceramide.

CONCLUSIONS: Long-term UV irradiation even with SED influences skin barrier function and structure with prominent ceramide decrease in SC intercellular lipid.
Full Text
http://onlinelibrary.wiley.com/doi/10.1111/j.1365-4632.2010.04789.x/abstract
DOI
10.1111/j.1365-4632.2010.04789.x
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Dermatology (피부과학교실) > 1. Journal Papers
Yonsei Authors
Lee, Seung Hun(이승헌)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/94342
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