Evaluation of two real-time PCR assays for the detection of Chlamydia trachomatis
이은영; 김신영; 김현숙
Journal of Laboratory Medicine and Quality Assurance (임상검사와 정도관리)
Journal of Laboratory Medicine and Quality Assurance (임상검사와 정도관리), Vol.33(1) : 43~47, 2011
BackgroundChlamydia trachomatis is common etiological agent of sexual transmitted disease. We evaluated two real-time PCR assays for the detection of C. trachomatis; Cobas TaqMan CT test v2.0 (Roche Diagnostics, Switzerland) and AdvanSure CT/NG real-time PCR (LG Life Sciences, Korea). And we compared performance of two real-time PCR assays with Cobas Amplicor CT test (Roche Diagnostics, Switzerland).Methods:Seventy-nine cervical swab specimens collected from patients with suspected C. trachomatis infection were tested using CTM CT, ADVANSURE, and AMPLICOR for the detection of C. trachomatis.
Results:Twenty-five (31.6%) samples showed positive results in all three assays. The positive and negative concordance rates among three assays were 100%
Conclusion:CTM CT and ADVANSURE showed excellent agreement with AMPLICOR. Therefore, these assays were reliable for the evaluation of C. trachomatis infection.