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Knock-down of human MutY homolog (hMYH) decreases phosphorylation of checkpoint kinase 1 (Chk1) induced by hydroxyurea and UV treatment

Title
 Knock-down of human MutY homolog (hMYH) decreases phosphorylation of checkpoint kinase 1 (Chk1) induced by hydroxyurea and UV treatment 
Authors
 Soo-Hyun Hahm ; Jong-Hwa Park ; Ye Sun Han ; Lin-Woo Kang ; Ji Hyung Chung ; In Sik Chung ; You Ri Lee ; Sung Il Ko 
Issue Date
2011
Journal Title
 BMB Reports 
ISSN
 1976-6696 
Citation
 BMB Reports, Vol.44(5) : 352~357, 2011 
Abstract
The effect of human MutY homolog (hMYH) on the activation of checkpoint proteins in response to hydroxyurea (HU) and ultraviolet (UV) treatment was investigated in hMYH-disrupted HEK293 cells. hMYH-disrupted cells decreased the phosphorylation of Chk1 upon HU or UV treatment and increased the phosphorylation of Cdk2 and the amount of Cdc25A, but not Cdc25C. In siMYH-transfected cells, the increased rate of phosphorylated Chk1 upon HU or UV treatment was lower than that in siGFP-transfected cells, meaning that hMYH was involved in the activation mechanism of Chk1 upon DNA damage. The phosphorylation of ataxia telangiectasia and Rad3- related protein (ATR) upon HU or UV treatment was decreased in hMYH-disrupted HEK293 and HaCaT cells. Co-immunoprecipitation experiments showed that hMYH was immunoprecipitated by anti-ATR. These results suggest that hMYH may interact with ATR and function as a mediator of Chk1 phosphorylation in response to DNA damage
URI
http://ir.ymlib.yonsei.ac.kr/handle/22282913/94149
DOI
10.5483/BMBRep.2011.44.5.352
Appears in Collections:
1. 연구논문 > 5. Research Institutes > Cardiovascular Product Evaluation Center
Yonsei Authors
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