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Knock-down of human MutY homolog (hMYH) decreases phosphorylation of checkpoint kinase 1 (Chk1) induced by hydroxyurea and UV treatment

Authors
 Soo-Hyun Hahm ; Jong-Hwa Park ; Ye Sun Han ; Lin-Woo Kang ; Ji Hyung Chung ; In Sik Chung ; You Ri Lee ; Sung Il Ko 
Citation
 BMB Reports, Vol.44(5) : 352~357, 2011 
Journal Title
 BMB Reports 
ISSN
 1976-6696 
Issue Date
2011
Abstract
The effect of human MutY homolog (hMYH) on the activation of checkpoint proteins in response to hydroxyurea (HU) and ultraviolet (UV) treatment was investigated in hMYH-disrupted HEK293 cells. hMYH-disrupted cells decreased the phosphorylation of Chk1 upon HU or UV treatment and increased the phosphorylation of Cdk2 and the amount of Cdc25A, but not Cdc25C. In siMYH-transfected cells, the increased rate of phosphorylated Chk1 upon HU or UV treatment was lower than that in siGFP-transfected cells, meaning that hMYH was involved in the activation mechanism of Chk1 upon DNA damage. The phosphorylation of ataxia telangiectasia and Rad3- related protein (ATR) upon HU or UV treatment was decreased in hMYH-disrupted HEK293 and HaCaT cells. Co-immunoprecipitation experiments showed that hMYH was immunoprecipitated by anti-ATR. These results suggest that hMYH may interact with ATR and function as a mediator of Chk1 phosphorylation in response to DNA damage
URI
http://ir.ymlib.yonsei.ac.kr/handle/22282913/94149
DOI
10.5483/BMBRep.2011.44.5.352
Appears in Collections:
1. 연구논문 > 5. Research Institutes > Cardiovascular Product Evaluation Center
Yonsei Authors
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