Recently, three-dimensional polymer scaffolds were developed for tissue regeneration. Conventional pre-wetting processes require a lengthy processing time and have residue problems. In this study, a helium atmospheric pressure glow discharge (He-APGD) was employed as a pre-wetting process for porous poly-(L-lactide-co-ɛ-caprolactone) (PLCL) scaffold. The three minutes treated PLCL scaffold absorbed the culture media and contained grafted hydroxyl, carbonyl, and carboxyl groups. Moreover, it did not show cytotoxicity, did not require residual rinsing steps and showed a homogeneous cell distribution. The He-APGD treatment has a short processing time and promotes a homogenous cell distribution. Therefore, this pre-wetting process is expected to find many applications in the future.