Cited 0 times in

Identification of oxidized serum albumin in the cerebrospinal fluid of ischaemic stroke patients

Authors
 G. J. Moon ; D. H. Shin ; B. J. Gwag ; K. Huh ; I. S. Joo ; J. H. Lee ; H. S. Nam ; O. Y. Bang ; D. S. Im 
Citation
 European Journal of Neurology, Vol.18(9) : 1151~1158, 2011 
Journal Title
 European Journal of Neurology 
ISSN
 1351-5101 
Issue Date
2011
Abstract
BACKGROUND AND PURPOSE:   Extensive evidence has shown that oxidative stress mediates neuronal death in animal models of hypoxic-ischaemia. Brain biomarkers of oxidative stress need to be identified in order to better understand and treat brain damage in human stroke patients. The present study was conducted to identify potential target proteins of oxidative stress in the cerebrospinal fluid (CSF) of stroke patients with acute ischaemic brain injury. METHODS:   We performed two-dimensional polyacrylamide gel electrophoresis to separate protein samples obtained from the CSF of control and stroke patients. To determine protein oxidation levels, oxyblot was then used to detect protein carbonyls that were determined by formation of a stable 2,4-dinitrophenylhydrazine (DNP) product using an anti-DNP antibody. RESULTS:   We found that oxidation of serum albumin was increased in the CSF from stroke patients as well as rats who underwent permanent middle cerebral artery occlusion (6.5%, 23%, respectively). In stroke patients, oxidized albumin levels correlated to neurologic indications. CONCLUSIONS:   The present study suggests that oxidized albumin in CSF can be utilized as an oxidative stress marker in human stroke patients
URI
http://ir.ymlib.yonsei.ac.kr/handle/22282913/93984
DOI
10.1111/j.1468-1331.2011.03357.x
Appears in Collections:
1. 연구논문 > 1. College of Medicine > Dept. of Neurology
Yonsei Authors
사서에게 알리기
  feedback
Link
 http://onlinelibrary.wiley.com/doi/10.1111/j.1468-1331.2011.03357.x/abstract
Export
RIS (EndNote)
XLS (Excel)
XML

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse