The expression of ERCC1, RRM1, and BRCA1 in breast cancer according to the immunohistochemical phenotypes.

223 440

Cited 31 times in

MeSH: Adult ; BRCA1 Protein/genetics* ; BRCA1 Protein/metabolism ; Biomarkers, Tumor/genetics* ; Biomarkers, Tumor/metabolism ; Breast Neoplasms/genetics* ; Breast Neoplasms/metabolism ; Breast Neoplasms/pathology ; DNA Repair ; DNA-Binding Proteins/genetics* ; DNA-Binding Proteins/metabolism ; Disease-Free Survival ; Endonucleases/genetics* ; Endonucleases/metabolism ; Female ; Gene Expression ; Humans ; Immunohistochemistry ; Middle Aged ; Phenotype ; Prognosis ; Protein Array Analysis ; Receptor, Epidermal Growth Factor/genetics ; Receptor, Epidermal Growth Factor/metabolism ; Tumor Suppressor Proteins/genetics* ; Tumor Suppressor Proteins/metabolism

Abstract: We studied the expression of BRCA1, ERCC1, and RRM1 which play an important role in DNA repair systems in breast cancer. Immunohistochemical staining for EGFR, BRCA1, ERCC1, and RRM1 were performed by using a tissue microarray made from 230 breast cancer patients. Patients were classified into luminal A, luminal B, HER-2, and triple negative breast cancer (TNBC) types according to ER, PR, and HER-2 expression. The expression of ERCC1, RRM1, and BRCA1 were correlated (P < 0.05). The expression level of ERCC1 was the lowest in TNBC type (P = 0.031), ERCC1 negativity was more prominent in TNBC and luminal B groups than luminal A and HER-2 groups (P = 0.013). Cases with EGFR overexpression showed high expression of RRM1 and BRCA1 (P = 0.046, and 0.004, respectively). In conclusion, the expression of ERCC1 is particularly lower in TNBCs than other types of breast cancers.