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Melatonin protects against oxidative stress in granular corneal dystrophy type 2 corneal fibroblasts by mechanisms that involve membrane melatonin receptors

Title
Melatonin protects against oxidative stress in granular corneal dystrophy type 2 corneal fibroblasts by mechanisms that involve membrane melatonin receptors
Authors
Seung-Il Choi;Shorafidinkhuja Dadakhujaev;Eung Kweon Kim;Tae im Kim;Hyunmi Ryu
Issue Date
2011
Journal Title
Journal of Pineal Research
ISSN
0742-3098
Citation
Journal of Pineal Research, Vol.51(1) : 94~103, 2011
Abstract
Considering that oxidative stress plays a role in corneal fibroblast degeneration during granular corneal dystrophy type 2 (GCD2) and melatonin is an effective antioxidant, we examined the ability of melatonin to protect against oxidative stress-induced cell death of primary cultured normal and GCD2-homozygous corneal fibroblasts. Melatonin treatment protected primary cultured normal and GCD2 corneal fibroblasts from paraquat (PQ)-induced oxidative stress and caused increased expression levels of Cu/Zn-superoxide dismutase (SOD1) and glutathione reductase (GR) in both types of cells. Interestingly, catalase expression increased in normal corneal fibroblasts, but decreased in GCD2 corneal fibroblasts after melatonin treatment. Melatonin also reduced the levels of intracellular reactive oxygen species and H(2)O(2) in both cell types. In addition, the selective melatonin receptor antagonist luzindole blocked melatonin-induced expression of SOD1 and GR. The expression levels of melatonin receptors 1A (MT1) and 1B (MT2) were significantly higher in GCD2 corneal fibroblasts than in normal cells. These results suggest that increased expression of melatonin receptors may be involved in the defense mechanisms against oxidative stress in GCD2 corneal fibroblasts, and melatonin may have potential therapeutic implications for GCD2 treatment.
URI
http://onlinelibrary.wiley.com/doi/10.1111/j.1600-079X.2011.00866.x/abstract

http://ir.ymlib.yonsei.ac.kr/handle/22282913/93493
DOI
10.1111/j.1600-079X.2011.00866.x
Appears in Collections:
1. 연구논문 > 5. Research Institutes > Corneal Dystrophy Research Institute
1. 연구논문 > 1. College of Medicine > Yonsei Biomedical Research Center
1. 연구논문 > 1. College of Medicine > Dept. of Ophthalmology
Yonsei Authors
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