460 670

Cited 29 times in

Nuclear localization of Nm23-H1 in head and neck squamous cell carcinoma is associated with radiation resistance.

Authors
 Haeng Ran Park  ;  Se-Heon Kim  ;  Sei Young Lee  ;  Ji Min Sung  ;  Ae Ran Park  ;  Suki Kang  ;  Baek Gil Kim  ;  Yoon Pyo Choi  ;  Yong-Bae Kim  ;  Nam Hoon Cho 
Citation
 CANCER, Vol.117(9) : 1864-1873, 2011 
Journal Title
CANCER
ISSN
 0008-543X 
Issue Date
2011
MeSH
Biomarkers, Tumor/analysis* ; Carcinoma,SquamousCell/enzymology ; Carcinoma,SquamousCell/genetics* ; Carcinoma,SquamousCell/mortality ; Carcinoma,SquamousCell/radiotherapy* ; CellLine, Tumor ; Disease-Free Survival ; HeadandNeckNeoplasms/enzymology ; HeadandNeckNeoplasms/genetics* ; HeadandNeckNeoplasms/mortality ; HeadandNeckNeoplasms/radiotherapy* ; Humans ; NM23 Nucleoside Diphosphate Kinases/genetics* ; Predictive Value of Tests ; RadiationTolerance*
Keywords
Nm23-H1 ; nuclear localization ; radiation ; resistance ; head and neck squamous cell carcinoma ; predictor
Abstract
BACKGROUND: Although radiation resistance is a primary issue in radiation therapy, attempts to find predictors of radiation resistance have met with little success. The authors therefore aimed to determine predictors for radiation resistance to improve the prognosis of head and neck squamous cell carcinoma (HNSCC).

METHODS: HNSCC cell lines, SCC15, SCC25, and QLL1, irradiated with an acute dose of 4 grays (Gy) (RR-4), a cumulative dose of 60 Gy (RR-60), and a booster dose of 4 Gy over 60 Gy (RR-60 + 4), were used with nonirradiated cell lines. Those were used in cDNA microarray, proteomics, Western blotting, and immunofluorescence, respectively. One hundred five HNSCC tissue samples with radiation resistance were analyzed by immunohistochemistry.

RESULTS: Western blot analysis of RR-60 cell lines was identical to the data of Nm23-H1 overexpression by cDNA array and proteomic screening. Immunofluorescence demonstrated significant nuclear translocation of Nm23-H1 in RR-4 and RR-60 cell lines, and less but still intense nuclear shuttling in RR-60 + 4. Similarly, Nm23-H1 nuclear localization was observed in 20% (21 of 105) of tissue samples. Univariate analysis demonstrated that Nm23-H1 nuclear localization was strongly associated with overall and recurrence-free survival. Multivariate stepwise Cox regression analysis showed that Nm23-H1 nuclear localization (odds ratio [OR], 7.48) and N stage (OR, 2.13) were associated with overall survival, and Nm23-H1 nuclear localization (OR, 3.02), T stage (OR, 1.43), and insufficient tumor margin (OR, 3.27) were associated with recurrence-free survival.

CONCLUSIONS: Overexpression of Nm23-H1, specifically its nuclear translocation, may be a powerful predictor of radiation resistance in HNSCC
Files in This Item:
T201101886.pdf Download
DOI
10.1002/cncr.25760
Appears in Collections:
1. College of Medicine (의과대학) > Research Institute (부설연구소) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Otorhinolaryngology (이비인후과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Radiation Oncology (방사선종양학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
Yonsei Authors
Kim, Baek Gil(김백길) ORCID logo https://orcid.org/0000-0001-6270-1433
Kim, Se Heon(김세헌)
Kim, Yong Bae(김용배) ORCID logo https://orcid.org/0000-0001-7573-6862
Park, Ae Ran(박애란)
Park, Haeng Ran(박행란)
Sung, Ji Min(성지민)
Cho, Nam Hoon(조남훈) ORCID logo https://orcid.org/0000-0002-0045-6441
Choi, Yoon Pyo(최윤표)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/93398
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse

Links