Cited 51 times in
Potential role of NADPH oxidase-mediated activation of Jak2/Stat3 and mitogen-activated protein kinases and expression of TGF-β1 in the pathophysiology of acute pancreatitis.
DC Field | Value | Language |
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dc.contributor.author | 김경환 | - |
dc.date.accessioned | 2014-12-20T16:47:01Z | - |
dc.date.available | 2014-12-20T16:47:01Z | - |
dc.date.issued | 2011 | - |
dc.identifier.issn | 1023-3830 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/93369 | - |
dc.description.abstract | OBJECTIVE: NADPH oxidase is potentially associated with acute pancreatitis by producing reactive oxygen species (ROS). We investigated whether NADPH oxidase mediates the activation of Janus kinase (Jak)2/signal transducers and activators of transcription (Stat)3 and mitogen-activated protein kinases (MAPKs) to induce the expression of transforming growth factor-β1 (TGF-β1) in cerulein-stimulated pancreatic acinar cells. TREATMENT: AR42J cells were treated with an NADPH oxidase inhibitor diphenyleneiodonium (DPI) or a Jak2 inhibitor AG490. Other cells were transfected with antisense or sense oligonucleotides (AS or S ODNs) for NADPH oxidase subunit p22(phox) or p47(phox). METHODS: TGF-β1 was determined by enzyme-linked immonosorbent assay. STAT3-DNA binding activity was measured by electrophoretic mobility shift assay. Levels of MAPKs as well as total and phospho-specific forms of Jak1/Stat3 were assessed by Western blot analysis. RESULTS: Cerulein induced increases in TGF-β1, Stat3-DNA binding activity and the activation of MAPKs in AR42J cells. AG490 suppressed these cerulein-induced changes, similar to inhibition by DPI. Cerulein-induced activation of Jak2/Stat3 and increases in MAPKs and TGF-β1 levels were inhibited in the cells transfected with AS ODN for p22(phox) and p47(phox) compared to S ODN controls. CONCLUSION: Inhibition of NADPH oxidase may be beneficial for prevention and treatment of pancreatitis by suppressing Jak2/Stat3 and MAPKs and expression of TGF-β1 in pancreatic acinar cells. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 791~800 | - |
dc.relation.isPartOf | INFLAMMATION RESEARCH | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Cell Line | - |
dc.subject.MESH | Ceruletide/pharmacology | - |
dc.subject.MESH | EnzymeActivation* | - |
dc.subject.MESH | Enzyme Inhibitors/metabolism | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Janus Kinase 2/metabolism* | - |
dc.subject.MESH | Mitogen-ActivatedProteinKinases/metabolism* | - |
dc.subject.MESH | NADPHOxidases/genetics | - |
dc.subject.MESH | NADPHOxidases/metabolism* | - |
dc.subject.MESH | Pancreas, Exocrine/cytology | - |
dc.subject.MESH | Pancreas, Exocrine/drug effects | - |
dc.subject.MESH | Pancreas, Exocrine/metabolism | - |
dc.subject.MESH | Pancreatitis/physiopathology* | - |
dc.subject.MESH | Rats | - |
dc.subject.MESH | Reactive Oxygen Species/metabolism | - |
dc.subject.MESH | STAT3Transcription Factor/metabolism* | - |
dc.subject.MESH | Transforming Growth Factor beta1/metabolism* | - |
dc.subject.MESH | Tyrphostins/metabolism | - |
dc.title | Potential role of NADPH oxidase-mediated activation of Jak2/Stat3 and mitogen-activated protein kinases and expression of TGF-β1 in the pathophysiology of acute pancreatitis. | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Pharmacology (약리학) | - |
dc.contributor.googleauthor | Kyung Don Ju | - |
dc.contributor.googleauthor | Joo Weon Lim | - |
dc.contributor.googleauthor | Kyung Hwan Kim | - |
dc.contributor.googleauthor | Hyeyoung Kim | - |
dc.identifier.doi | 10.1007/s00011-011-0335-4 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00311 | - |
dc.relation.journalcode | J01059 | - |
dc.identifier.eissn | 1420-908X | - |
dc.identifier.pmid | 21509626 | - |
dc.identifier.url | http://link.springer.com/article/10.1007%2Fs00011-011-0335-4 | - |
dc.subject.keyword | Cerulein | - |
dc.subject.keyword | NADPH oxidase | - |
dc.subject.keyword | Jak2/Stat3 | - |
dc.subject.keyword | TGF-β1 | - |
dc.subject.keyword | Pancreatic acinar cells | - |
dc.contributor.alternativeName | Kim, Kyung Hwan | - |
dc.contributor.affiliatedAuthor | Kim, Kyung Hwan | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 60 | - |
dc.citation.number | 8 | - |
dc.citation.startPage | 791 | - |
dc.citation.endPage | 800 | - |
dc.identifier.bibliographicCitation | INFLAMMATION RESEARCH, Vol.60(8) : 791-800, 2011 | - |
dc.identifier.rimsid | 27167 | - |
dc.type.rims | ART | - |
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