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Enhanced adipogenic differentiation and reduced collagen synthesis induced by human periodontal ligament stem cells might underlie the negative effect of recombinant human bone morphogenetic protein-2 on periodontal regeneration.

Title
 Enhanced adipogenic differentiation and reduced collagen synthesis induced by human periodontal ligament stem cells might underlie the negative effect of recombinant human bone morphogenetic protein-2 on periodontal regeneration.
Authors
 D-S. Song; J-C. Park; C-S. Kim; C-K. Kim; K-S. Cho; S-H. Choi; I-H. Jung
Issue Date
2011
Journal Title
 Journal of Periodontal Research
ISSN
 0022-3484
Citation
 Journal of Periodontal Research, Vol.46(2) : 193~203, 2011
Abstract
BACKGROUND AND OBJECTIVE: Recombinant human bone morphogenetic protein-2 (rhBMP-2) is a potent inducer for the regeneration of mineralized tissue, but has a limited effect on the regeneration of cementum and periodontal ligament (PDL). The aim of the present study was to determine the effects of rhBMP-2 on the in vitro and in vivo biologic activity of well-characterized human PDL stem cells (hPDLSCs) and to elucidate the underlying mechanism of minimal periodontal regeneration by rhBMP-2. MATERIAL AND METHODS: hPDLSCs were isolated and cultured, and then transplanted into an ectopic subcutaneous mouse model using a carrier treated either with or without rhBMP-2. Comprehensive histologic, histometric and immunohistochemical analyses were performed after an 8-wk healing period. The effects of rhBMP-2 on the adipogenic and osteogenic/cementogenic differentiation of hPDLSCs were also evaluated. The effect of rhBMP-2 on both soluble and insoluble collagen synthesis was analyzed, and the expression of mRNA and protein for collagen types I, II, III and V was assessed. RESULTS: In the present study, rhBMP-2 promoted both adipogenic and osteogenic/cementogenic differentiation of hPDLSCs in vitro, and the in vivo potential of hPDLSCs to form mineralized cementum and organized PDL tissue was down-regulated following treatment with rhBMP-2. Collagen synthesis, which plays a crucial role in the regeneration of cementum and the periodontal attachment, was significantly reduced, with associated modification of the relevant mRNA and protein expression profiles. CONCLUSION: In summary, the findings of the present study suggest that enhanced adipogenic differentiation and inhibition of collagen synthesis by hPDLSCs appear to be partly responsible for the minimal effect of rhBMP-2 on cementum and PDL tissue regeneration by hPDLSCs
URI
http://ir.ymlib.yonsei.ac.kr/handle/22282913/93308
DOI
10.1111/j.1600-0765.2010.01328.x
Appears in Collections:
1. 연구논문 > 5. Research Institutes > Research Center for Orofacial Hard Tissue Regeneration
1. 연구논문 > 2. College of Dentistry > Dept. of Periodontology
Yonsei Authors
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Link
 http://onlinelibrary.wiley.com/doi/10.1111/j.1600-0765.2010.01328.x/abstract;jsessionid=98DA3B7386C465187F870A20CFC10028.f01t02
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