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Calpain mediates degradation of cytoskeletal proteins during Jurkat T-cell death induced by Entamoeba histolytica

Authors
 Y. A. LEE ; K. A. KIM ; M. H. SHIN 
Citation
 Parasite Immunology, Vol.33(6) : 349~356, 2011 
Journal Title
 Parasite Immunology 
ISSN
 0141-9838 
Issue Date
2011
Abstract
Entamoeba histolytica is known to induce host cell death via activation of calpain and caspases. In this study, we investigated the specific proteases involved in the degradation of cytoskeletal proteins during Jurkat T-cell death induced by E. histolytica. Amoebic trophozoites induced marked degradation of paxillin, Cas, vimentin, vinculin and talin, as well as α- or β-spectrin, in Jurkat T cells. The cleavage effects of E. histolytica were strongly retarded by pretreatment with a calpain inhibitor, but not with a pan-caspase inhibitor. In addition, calpain knockdown with siRNA in Jurkat T cells effectively inhibited E. histolytica-induced PARP, paxillin, α-spectrin, β-spectrin and talin degradation, as compared to scrambled siRNA. These results suggest that calpain plays a crucial role in the cleavage of cytoskeletal proteins during cell death induced by E. histolytica.
URI
http://ir.ymlib.yonsei.ac.kr/handle/22282913/93296
DOI
10.1111/j.1365-3024.2011.01290.x
Appears in Collections:
1. 연구논문 > 1. College of Medicine > Dept. of Environmental Medical Biology
Yonsei Authors
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Link
 http://onlinelibrary.wiley.com/doi/10.1111/j.1365-3024.2011.01290.x/abstract
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