RASSF1A suppresses the activated K-Ras-induced oxidative DNA damage.
Seon Ho Park ; Jung Jin Kim ; Young Do Yoo ; Hyung Jung Kim ; Gi Young Lee ; So Ra Lee ; Jin Sil Chung
Biochemical and Biophysicial Research Communications, Vol.408(1) : 149~153, 2011
Biochemical and Biophysicial Research Communications
The mutant K-Ras elevates intracellular reactive oxygen species (ROS) levels and leads to oxidative DNA damage, resulting in malignant cell transformation. Ras association domain family 1 isoform A (RASSF1A) is known to play a role as a Ras effector. However, the suppressive effect of RASSF1A on K-RasV12-induced ROS increase and DNA damage has not been identified. Here, we show that RASSF1A blocks K-RasV12-triggered ROS production. RASSF1A expression also inhibits oxidative DNA damage and chromosomal damage. From the results obtained in this study, we suggest that RASSF1A regulates the cellular ROS levels enhanced by the Ras signaling pathway, and that it may function as a tumor suppressor by suppressing DNA damage caused by activated Ras.