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Bone formation of Escherichia coli expressed rhBMP-2 on absorbable collagen block in rat calvarial defects

Authors
 Jee-Hee Jung  ;  Jeong-Ho Yun  ;  Yoo-Jung Um  ;  Ui-Won Jung  ;  Chang-Sung Kim  ;  Seong-Ho Choi  ;  Kyoo-Sung Cho 
Citation
 ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY AND ENDODONTICS, Vol.111(3) : 298-305, 2011 
Journal Title
ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY AND ENDODONTICS
ISSN
 1079-2104 
Issue Date
2011
MeSH
Absorbable Implants* ; Animals ; Biocompatible Materials/therapeutic use* ; Bone Marrow/pathology ; Bone Matrix/pathology ; Bone Morphogenetic Protein 2 ; Bone Morphogenetic Proteins/therapeutic use ; Bone Regeneration/physiology ; Calcification, Physiologic/physiology ; Collagen/therapeutic use* ; Connective Tissue/pathology ; Disease Models, Animal ; Drug Carriers ; Escherichia coli* ; Image Processing, Computer-Assisted/methods ; Male ; Osteoblasts/pathology ; Osteocytes/pathology ; Osteogenesis/physiology* ; Rats ; Rats, Sprague-Dawley ; Recombinant Proteins/therapeutic use ; Skull/pathology ; Skull/surgery* ; Time Factors ; Transforming Growth Factor beta/therapeutic use
Abstract
OBJECTIVES: The aim of this study was to evaluate the osteogenic and space-providing effect of rhBMP-2 produced in an Escherichia coli expression system (ErhBMP-2) with absorbable collagen block (ACB) as the carrier system in the rat calvarial defect model.

STUDY DESIGN: Eight-millimeter diameter calvarial defects were created in 60 male Sprague-Dawley rats. The animals were divided into 6 groups containing 10 animals each that received sham-surgery control (no material applied), ACB control (ACB alone), or ErhBMP-2/ACB (ACB loaded with 0.025 mg/mL ErhBMP-2). Histological and histometric analysis was performed after 2- and 8-week healing intervals.

RESULTS: On histological observation, the level of bone formation in the defects was generally higher at 8 weeks than at 2 weeks. Surgical implantation of ErhBMP-2/ACB resulted in the enhanced bone regeneration compared with controls. Moreover, the collagen remnants of ACB appeared to be completely resorbed in ACB control and ErhBMP-2/ACB group after 8 weeks. Histometic analysis revealed that the ErhBMP-2/ACB group had a significantly greater augmented area, new bone area, and new bone ratio after 2 and 8 weeks than both control groups. However, there was no difference between 2 and 8 weeks in the ErhBMP-2/ACB group in all aspects of the augmented area, new bone area, and new bone ratio (P < .05).

CONCLUSION: ErhBMP-2 loaded on ACB can induce favorable bone formation in the rat calvarial defect model, but this collagen carrier in block type did not fulfill the space-maintaining expectations for bone formation by ErhBMP-2.
Full Text
http://www.sciencedirect.com/science/article/pii/S1079210410003239
DOI
10.1016/j.tripleo.2010.05.011
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Periodontics (치주과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Chang Sung(김창성) ORCID logo https://orcid.org/0000-0003-3902-1071
Um, Yoo Jung(엄유정)
Yun, Jeong Ho(윤정호)
Jung, Ui Won(정의원) ORCID logo https://orcid.org/0000-0001-6371-4172
Cho, Kyoo Sung(조규성) ORCID logo https://orcid.org/0000-0002-6777-5287
Choi, Seong Ho(최성호) ORCID logo https://orcid.org/0000-0001-6704-6124
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/92926
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