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Effects of agmatine on hypoxic microglia and activity of nitric oxide synthase

Title
Effects of agmatine on hypoxic microglia and activity of nitric oxide synthase
Authors
Soo Kyung Ahn;Samin Hong;Jong Eun Lee;Kyung Ah Park;Won Taek Lee;Yu Mi Park
Issue Date
2011
Journal Title
Brain Research
ISSN
0006-8993
Citation
Brain Research, Vol.1373(null) : 48~54, 2011
Abstract
Microglia are the resident macrophages of CNS and play a crucial role in maintaining homeostasis against various neuronal injuries. However, excessive activation of microglia may destroy healthy neurons as well as damaged neurons. We investigated neuroprotective effects of amgatine on hypoxic microglia using in vitro and in vivo models for transient hypoxia. For in vitro study, BV2 immortalized murine microglia were incubated with or without 100 μM of agmatine in a closed anaerobic chamber for 2h. After recovery in normoxic condition for 20 h, cell viability and the amount of nitrite generation were determined. For in vivo study, 100mg/kg of agmatine or equivalent volume of saline was intraperitoneally administered, and the left middle cerebral artery of adult male Sprague-Dawley rats was occluded for 90 min. After 24h from occlusion, the cortex and striatum of the forebrains was evaluated to check the immunoreactivity with a microglial marker, ionized calcium binding adaptor molecule 1 (Iba1), and inducible nitric oxide synthase (iNOS). Results showed that agmatine attenuated hypoxia-induced cytotoxicity and nitrite production by BV2 microglia. Agmatine also decreased the activities of microglia and NOS induced by transient middle cerebral artery occlusion. Finally, our findings reveal that agmatine may reduce microglial damages caused by transient hypoxia and suggest that agmatine may lead to a novel therapeutic strategy for hypoxic neuronal injuries.
URI
http://www.sciencedirect.com/science/article/pii/S0006899310026132

http://ir.ymlib.yonsei.ac.kr/handle/22282913/92688
DOI
10.1016/j.brainres.2010.12.002
Appears in Collections:
1. 연구논문 > 1. College of Medicine > Dept. of Ophthalmology
1. 연구논문 > 1. College of Medicine > Dept. of Anatomy
1. 연구논문 > 1. College of Medicine > Dept. of Internal Medicine
Yonsei Authors
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