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Cardiomyocytes from phorbol myristate acetate-activated mesenchymal stem cells restore electromechanical function in infarcted rat hearts

Authors
 Heesang Song ; Hye Jin Hwang ; Ki-Chul Hwang ; Yangsoo Jang ; Sung-Hou Kim ; Moon-Hyoung Lee ; Myoungho Lee ; Chungkeun Lee ; Eunmi Choi ; Se-Yeon Lee ; Jun-Hee Park ; Chang Youn Lee ; Onju Ham ; Eun Ju Choi ; Soyeon Lim ; Il-Kwon Kim ; Min-Ji Cha ; Byeong-Wook Song ; Woochul Chang 
Citation
 Proceedings of the National Academy of Sciences of the United States of America, Vol.108(1) : 296~301, 2011 
Journal Title
 Proceedings of the National Academy of Sciences of the United States of America 
ISSN
 0027-8424 
Issue Date
2011
Abstract
Despite the safety and feasibility of mesenchymal stem cell (MSC) therapy, an optimal cell type has not yet emerged in terms of electromechanical integration in infarcted myocardium. We found that poor to moderate survival benefits of MSC-implanted rats were caused by incomplete electromechanical integration induced by tissue heterogeneity between myocytes and engrafted MSCs in the infarcted myocardium. Here, we report the development of cardiogenic cells from rat MSCs activated by phorbol myristate acetate, a PKC activator, that exhibited high expressions of cardiac-specific markers and Ca(2+) homeostasis-related proteins and showed adrenergic receptor signaling by norepinephrine. Histological analysis showed high connexin 43 coupling, few inflammatory cells, and low fibrotic markers in myocardium implanted with these phorbol myristate acetate-activated MSCs. Infarct hearts implanted with these cells exhibited restoration of conduction velocity through decreased tissue heterogeneity and improved myocardial contractility. These findings have major implications for the development of better cell types for electromechanical integration of cell-based treatment for infarcted myocardium.
URI
http://ir.ymlib.yonsei.ac.kr/handle/22282913/92669
DOI
10.1073/pnas.1015873107/-/DCSupplemental
Appears in Collections:
1. 연구논문 > 1. College of Medicine > Dept. of Life Science
1. 연구논문 > 1. College of Medicine > Dept. of Internal Medicine
1. 연구논문 > 1. College of Medicine > Medical Research Center
1. 연구논문 > 1. College of Medicine > Yonsei Biomedical Research Center
Yonsei Authors
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